The Isolation and Replication of African Swine Fever Virus in Primary Renal-Derived Swine Macrophages

被引:11
|
作者
Oh, Taehwan [1 ]
Do, Duy Tien [2 ]
Vo, Hung Van [3 ]
Kwon, Hyeok-il [4 ]
Lee, Seung-Chul [4 ]
Kim, Min Ho [4 ]
Nguyen, Dung Thi Thu [2 ]
Le, Quang Tin Vinh [3 ]
Tran, Tan Minh [3 ]
Nguyen, Toan Tat [2 ]
Lee, Joo Young [4 ]
Chae, Chanhee [1 ]
机构
[1] Seoul Natl Univ, Dept Vet Pathol, Coll Vet Med, Seoul, South Korea
[2] Nong Lam Univ, Fac Anim Sci & Vet Med, Ho Chi Minh City, Vietnam
[3] Reg Anim Hlth Off 6, Dept Anim Hlth, Ctr Vet Diagnost, Ho Chi Minh City, Vietnam
[4] ChoongAng Vaccine Labs, Daejeon, South Korea
关键词
virus replication; virus isolation; African swine fever; molecular chacterization; renal-derived macrophages; RESPIRATORY SYNDROME VIRUS; DIFFERENTIATION; SUSCEPTIBILITY; MONOCYTES/MACROPHAGES; PATHOGENESIS; EXPRESSION; INFECTION; CD163; PIGS;
D O I
10.3389/fvets.2021.645456
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
African swine fever virus (ASFV) causes hemorrhagic disease in domestic pigs by replicating mainly in monocyte/macrophage lineages. Various primary cells including pulmonary alveolar macrophages have been used for the propagation of ASFV on this account. However, ethical constraints and consistency problems exist as it is necessary to harvest same phenotype of primary cells in order to continue a study. We suggested renal-derived swine macrophages as a novel primary cell candidate to address these issues. These primary cells proved to be permissive to both cell adapted ASFV and a wild-type ASFV. Compared to the commercial cell line MA-104, the renal-derived macrophages were more suitable to isolate the field virus. The consistent molecular characteristics of the renal-derived macrophages were demonstrated by immunocytochemistry with antibodies against macrophage cell surface markers including CD163, CD172a, and Iba-1. Viral protein p30 and p72 expression in ASFV infected macrophages was confirmed by immunocytochemistry by use of specific monoclonal antibodies. We observed increase of cell-free viral DNA and infectious virus titer in infected cell supernatant in successive days-post-infection. These results demonstrated that primary renal-derived swine macrophages are useful for ASFV isolation and propagation in terms of cell phenotypes, susceptibility to the virus, and virus production.
引用
收藏
页数:7
相关论文
共 50 条
  • [41] The CD2v protein enhances African swine fever virus replication in the tick vector, Ornithodoros erraticus
    Rowlands, Rebecca J.
    Duarte, Margarida M.
    Boinas, Fernando
    Hutchings, Geoff
    Dixon, Linda K.
    VIROLOGY, 2009, 393 (02) : 319 - 328
  • [42] African Swine Fever Virus Exhibits Distinct Replication Defects in Different Cell Types
    Gao, Yanni
    Xia, Tingting
    Bai, Juan
    Zhang, Lujie
    Jiang, Xiaolin
    Yang, Xing
    Zhang, Keshan
    Jiang, Ping
    VIRUSES-BASEL, 2022, 14 (12):
  • [43] Disinfection to control African swine fever virus: a UK perspective
    Wales, Andrew D.
    Davies, Robert H.
    JOURNAL OF MEDICAL MICROBIOLOGY, 2021, 70 (09)
  • [44] Estimation of the transmission dynamics of African swine fever virus within a swine house
    Nielsen, J. P.
    Larsen, T. S.
    Halasa, T.
    Christiansen, L. E.
    EPIDEMIOLOGY AND INFECTION, 2017, 145 (13): : 2787 - 2796
  • [45] Comparative inhibitory activity of the stilbenes resveratrol and oxyresveratrol on African swine fever virus replication
    Galindo, I.
    Hernaez, B.
    Berna, J.
    Fenoll, J.
    Cenis, J. L.
    Escribano, J. M.
    Alonso, C.
    ANTIVIRAL RESEARCH, 2011, 91 (01) : 57 - 63
  • [46] ASF -survivors' sera do not inhibit African swine fever virus replication in vitro
    Walczak, Marek
    Juszkiewicz, Malgorzata
    Szymankiewicz, Krzesimir
    Szczotka-Bochniarz, Anna
    Wozniakowski, Grzegorz
    JOURNAL OF VETERINARY RESEARCH, 2022, 66 (01) : 21 - 27
  • [47] Salt inactivation of classical swine fever virus and African swine fever virus in porcine intestines confirms the existing in vitro casings model
    Jelsma, Tinka
    Wijnker, Joris J.
    Smid, Bregtje
    Verheij, Eline
    van der Poel, Wim H. M.
    Wisselink, Henk J.
    VETERINARY MICROBIOLOGY, 2019, 238
  • [48] African swine fever
    Penrith, Mary-Louise
    ONDERSTEPOORT JOURNAL OF VETERINARY RESEARCH, 2009, 76 (01) : 91 - 95
  • [49] African swine fever
    Dixon, L. K.
    Sun, H.
    Roberts, H.
    ANTIVIRAL RESEARCH, 2019, 165 : 34 - 41
  • [50] Cell Lines for the Development of African Swine Fever Virus Vaccine Candidates: An Update
    Meloni, Dionigia
    Franzoni, Giulia
    Oggiano, Annalisa
    VACCINES, 2022, 10 (05)