Physicochemical Properties of Photoconvertible Fluorescent Protein from Montastraea cavernosa

被引：1

作者：

Frolova, A. Yu ^{[1
,2
]}

Pakhomov, A. A. ^{[1
,2
]}

Martynov, V., I ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Nesmeyanov Inst Organoelement Cpds, Moscow 119334, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2021年 / 47卷 / 01期

基金：

俄罗斯基础研究基金会; 俄罗斯科学基金会;

关键词：

chromophore; photoconversion; fluorescent protein; mcavGR; mcavRFP; dendFP; Dendra2; TO-RED CONVERSION; GREEN; EVOLUTION; DYNAMICS; TRACKING; CELL; DIVERSITY; MECHANISM; VERSION; BRIGHT;

D O I：

10.1134/S1068162021010052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Among the wide variety of currently known fluorescent probes, photoconvertible fluorescent proteins (PCFPs), which are capable to irreversibly change their maximum of fluorescence emission under specific light irradiation, are of special interest. In this work, we have studied the basic physicochemical properties of PCFP isolated from Montastraea cavernosa (mcavGR). It has been shown that mcavGR demonstrates both high brightness and photoconversion contrast, which is comparable with the best homologous PCFPs, such as Kaede, dendFP, Dendra2, EosFP, etc., and at the same time has significant photostability.

引用

页码：244 / 251

页数：8

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