Isolation of adipose-derived stem cells by using a subfractionation culturing method

被引:22
作者
Yi, TacGhee [1 ,2 ,3 ]
Kim, Wang-Kyun [4 ,5 ]
Choi, Joon-Seok [6 ]
Song, Seung Yong [7 ]
Han, Juhee [4 ,5 ]
Kim, Ji Hye [4 ,5 ]
Kim, Won-Serk [8 ]
Park, Sang Gyu [9 ]
Lee, Hyun-Joo [3 ,10 ]
Cho, Yun Kyoung [3 ]
Hwang, Sung-Joo [5 ]
Song, Sun U. [1 ,2 ,3 ]
Sung, Jong-Hyuk [5 ]
机构
[1] Inha Univ, Sch Med, Translat Res Ctr, Inchon 400711, South Korea
[2] Inha Res Inst Med Sci, Inchon 400711, South Korea
[3] SCM Lifesci Co Ltd, Inchon, South Korea
[4] CHA Univ, Dept Appl Biosci, Seoul, South Korea
[5] Yonsei Univ, Coll Pharm, Inchon 406840, South Korea
[6] Seoul Natl Univ, Inst Entrepreneurial BioConvergence, Seoul, South Korea
[7] Yonsei Univ, Coll Med, Dept Plast & Reconstruct Surg, Seoul, South Korea
[8] Sungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Dept Dermatol, Seoul, South Korea
[9] CHA Univ, Dept Biomed Sci, Seoul, South Korea
[10] Inha Univ, Sch Med, Dept Med, Drug Dev Program, Inchon 400711, South Korea
基金
新加坡国家研究基金会;
关键词
adipose-derived stem cell; hair regeneration; isolation; proliferation; subfractionation culturing method; BONE-MARROW; TISSUE; DIFFERENTIATION; GENERATION; PROLIFERATION; THERAPY; CD146; SKIN;
D O I
10.1517/14712598.2014.943661
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Objective: Adipose-derived stem cells (ASCs) isolated from subcutaneous adipose tissue have been tested in clinical trials. However, ASCs isolated by enzyme digestion and centrifugation are heterogeneous and exhibit wide variation in regenerative potential and clinical outcomes. Therefore, we developed a new method for isolating clonal ASCs (cASCs) that does not use enzyme digestion or centrifugation steps. Research design and methods: In addition to cell surface markers and differentiation potential, we compared the mitogenic, paracrine and hair growth-promoting effects of ASCs isolated by the gradient centrifugation method (GCM) or by the new subfractionation culturing method (SCM). Results: We selected three cASCs isolated by SCM that showed high rates of proliferation. The cell surface markers expressed by ASCs isolated by GCM or SCM were very similar, and SCM-isolated ASCs could potentially differentiate into different cell lineages. However, cASC lines exhibited better mitogenic and paracrine effects than ASCs isolated by GCM. The expression of Diras3, Myb, Cdca7, Mki67, Rrm2, Cdk1 and Ccna2, which may play a key role in cASC proliferation, was upregulated in cASCs. In addition, cASCs exhibited enhanced hair growth-promoting effects in dermal papilla cells and animal experiments. Conclusions: SCM generates a highly homogeneous population of ASCs via a simple and effective procedure that can be used in therapeutic settings.
引用
收藏
页码:1551 / 1560
页数:10
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