Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of 6-benzylaminopurine and its ribose adduct in bean sprouts

被引：23

作者：

Zhang, Wei ^{[1
]}

He, Lishan ^{[1
]}

Zhang, Rui ^{[1
]}

Guo, Suqin ^{[1
]}

Yue, Huanfang ^{[1
]}

Ning, Xiangxue ^{[1
]}

Tan, Guiyu ^{[1
]}

Li, Qing X. ^{[2
]}

Wang, Baomin ^{[1
]}

机构：

[1] China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China

[2] Univ Hawaii Manoa, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA

来源：

FOOD CHEMISTRY | 2016年 / 207卷

关键词：

6-Benzylaminopurine; Monoclonal antibody; ELISA; Food safety; Plant growth regulator; CELL-DIVISION; PLANT-TISSUES; REGULATORS; METABOLISM; 6-BENZYLADENINE; IDENTIFICATION; CULTURES;

D O I：

10.1016/j.foodchem.2016.03.103

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

6-Benzylaminopurine (6-BA), a cytokinin plant growth regulator, has been banned for use in bean sprout production in China. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with a specific monoclonal antibody (mAb 3E5). The assay showed a half-maximum inhibition concentration (IC50) and detection range of 18.9 ng/mL and 3.6-106 ng/mL, respectively. Recoveries of 6-BA spiked in home cultured bean sprout samples averaged from 75% to 89% with a correlation coefficient (R-2) of 0.998 between the results determined by icELISA and those by liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry (LC-ESI-MS). LC-ESI-MS showed that 6-BA had been partially metabolized to 6-benzylaminopurine riboside (6-BAR) in the positive samples. The content of 6-BA determined by icELISA was about 5-70 times higher than that of LC-ESI-MS because mAb 3E5 had 315% cross-reactivity with 6-BAR. Such icELISA being ultra-sensitive to 6-BAR would allow quick monitoring of 6-BA by detecting 6-BAR as a potential biomarker. (C) 2016 Published by Elsevier Ltd.

引用

页码：233 / 238

页数：6

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