PARP Activity and NAD Concentration in PMC from Patients Affected by Systemic Sclerosis and Lupus Erythematosus

被引：7

作者：

Cerboni, B. ^{[1
]}

Di Stefano, A. ^{[1
]}

Micheli, V. ^{[1
]}

Morozzi, G. ^{[2
]}

Pompucci, G. ^{[1
]}

Sestini, S. ^{[1
]}

机构：

[1] Univ Siena, Sect Biol Chem, Dept Mol Biol, I-53100 Siena, Italy

[2] Univ Siena, Div Rheumatol, Clin Med & Immunol Sci Dept, I-53100 Siena, Italy

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2010年 / 29卷 / 4-6期

关键词：

NAD; PARP-1; SLE; systemic sclerosis; HUMAN-LYMPHOCYTES; POLY(ADP-RIBOSE); AUTOANTIBODIES; POLYMERASE;

D O I：

10.1080/15257771003741471

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme poly(ADP-ribose) polymerase (PARP-1, EC 2.4.2.30) is activated by DNA strand breaks caused by several agents and utilizes NAD to form polyADPR, bound to acceptor proteins. The involvement of PARP-1 in autoimmune diseases has been suggested: antiPARP autoantibodies are described in systemic lupus erythematosus (SLE), DNA strand breaks have been evidenced in systemic sclerosis (SSc). We tested poly(ADP-ribosyl)ation activity and NAD concentration in PMC from patients affected by SLE or SSc and from controls. Lower PARP-1 activity and higher NAD concentration were observed in pathological conditions than controls, supporting the role of PARP-1 activation in modulating NAD concentration.

引用

页码：471 / 475

页数：5

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