Helicobacter pylori from Peptic Ulcer Patients in Uganda Is Highly Resistant to Clarithromycin and Fluoroquinolones: Results of the GenoType HelicoDR Test Directly Applied on Stool

被引:12
作者
Angol, Denish Calmax [1 ,2 ]
Ocama, Ponsiano [3 ]
Kirabo, Tess Ayazika [1 ]
Okeng, Alfred [4 ]
Najjingo, Irene [4 ]
Bwanga, Freddie [1 ,4 ]
机构
[1] Makerere Univ, Coll Hlth Sci, Dept Med Microbiol, POB 7072, Kampala, Uganda
[2] Lira Univ, Dept Midwifery, Fac Hlth Sci, POB 1035, Lira, Uganda
[3] Makerere Univ, Dept Med, Coll Hlth Sci, POB 7072, Kampala, Uganda
[4] MBN Clin Labs, Plot 28 Nakasero Rd, Kampala, Uganda
关键词
REAL-TIME PCR; ANTIBIOTIC-RESISTANCE; ANTIMICROBIAL RESISTANCE; SUSCEPTIBILITY; EPIDEMIOLOGY; PREVALENCE; INFECTION; DIAGNOSIS; CHILDREN; PATHOGENESIS;
D O I
10.1155/2017/5430723
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background. Around 70-90% of peptic ulcer disease (PUD) is due to Helicobacter pylori and requires treatment with antimicrobials to which these bacteria are susceptible. Common H. pylori diagnostic tests do not provide drug susceptibility data. Using the GenoType HelicoDR PCR test designed for gastric biopsies for simultaneous detection of H. pylori and its resistance to clarithromycin (CLA)/fluoroquinolones (FLQ), we present evidence for stool as an optional test specimen and also provide data on prevalence of H. pylori resistance to CLA and FLQ in Uganda. Methods. Stool from 142 symptomatic PUD patients at three hospitals in Kampala was screened for H. pylori using a rapid antigen test. The GenoType HelicoDR test was run on all H. pylori antigen positives to determine PCR positivity and resistance to CLA/FLQ. Results. Thirty-one samples (22%) were H. pylori antigen positive, and 21 (68%) of these were H. pylori PCR positive. Six of the 21 (29%) were resistant to CLA and eight to FLQ (42%), while two gave invalid FLQ resistance results. Conclusion. Stool is a possible specimen for the GenoType HelicoDR test for rapid detection of H. pylori and drug resistance. In Uganda, Helicobacter pylori is highly resistant to CLA and FLQ.
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页数:10
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