Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila

被引：52

作者：

Anduaga, Ane Martin ^{[1
]}

Evantal, Naveh ^{[2
]}

Patop, Ines Lucia ^{[1
]}

Bartok, Osnat ^{[2
]}

Weiss, Ron ^{[2
]}

Kadener, Sebastian ^{[1
,2
]}

机构：

[1] Brandeis Univ, Dept Biol, Waltham, MA 02254 USA

[2] Hebrew Univ Jerusalem, Silberman Inst Life Sci, Jerusalem, Israel

来源：

ELIFE | 2019年 / 8卷

基金：

美国国家卫生研究院;

关键词：

CIRCADIAN GENE-EXPRESSION; POSTTRANSCRIPTIONAL CONTROL; CLOCK GENE; MOLECULAR PHYLOGENY; BEHAVIORAL RHYTHMS; PERIOD; LIGHT; ORGANIZATION; OSCILLATIONS; MECHANISMS;

D O I：

10.7554/eLife.44642

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Circadian rhythms are generated by the cyclic transcription, translation, and degradation of clock gene products, including timeless (tim), but how the circadian clock senses and adapts to temperature changes is not completely understood. Here, we show that temperature dramatically changes the splicing pattern of tim in Drosophila. We found that at 18 degrees C, TIM levels are low because of the induction of two cold-specific isoforms: tim-cold and tim-short and cold. At 29 degrees C, another isoform, tim-medium, is upregulated. Isoform switching regulates the levels and activity of TIM as each isoform has a specific function. We found that tim-short and cold encodes a protein that rescues the behavioral defects of tim(01) mutants, and that flies in which tim-short and cold is abrogated have abnormal locomotor activity. In addition, miRNA-mediated control limits the expression of some of these isoforms. Finally, data that we obtained using minigenes suggest that tim alternative splicing might act as a thermometer for the circadian clock.

引用

页数：31

共 93 条

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