Ion channel behavior of supported bilayer lipid membranes on a glassy carbon electrode

被引:56
|
作者
Wu, ZY
Tang, JL
Cheng, ZL
Yang, XR
Wang, EK [1 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, Electroanalyt Chem Lab, Changchun 130022, Peoples R China
[2] Chinese Acad Sci, Changchun Inst Appl Chem, Natl Analyt Res Ctr Electrochem & Spect, Changchun 130022, Peoples R China
关键词
D O I
10.1021/ac000764x
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new kind of solid substrate, a glassy carbon (GC) electrode, was selected to support lipid layer membranes. On the surface of the GC electrode, we made layers of didodecyldimethylammonium bromide (a synthetic lipid). From electrochemical impedance experiments, we demonstrated that the lipid layers on the GC electrode were bilayer lipid membranes. We studied the ion channel behavior of the supported bilayer lipid membrane. In the presence of perchlorate anions as the stimulus and ruthenium(II) complex cations as the marker ions, the lipid membrane channel was open and exhibited distinct channel current. The channel was in a closed state in the absence of perchlorate anions.
引用
收藏
页码:6030 / 6033
页数:4
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