The PHD Transcription Factor Rum1 Regulates Morphogenesis and Aflatoxin Biosynthesis in Aspergillus flavus

被引：33
作者：
Hu, Yule [1 ,2 ]
Yang, Guang [1 ,2 ]
Zhang, Danping [1 ,2 ]
Liu, Yaju [1 ,2 ]
Li, Yu [1 ,2 ,3 ]
Lin, Guanglan [1 ,2 ]
Guo, Zhiqiang [1 ,2 ]
Wang, Shihua [1 ,2 ]
Zhuang, Zhenhong [1 ,2 ]
机构：
[1] Fujian Agr & Forestry Univ, Educ Minist, Key Lab Biopesticide & Chem Biol, Fujian Key Lab Pathogen Fungi & Mycotoxins, Fuzhou 350002, Fujian, Peoples R China
[2] Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Fujian, Peoples R China
[3] Xiamen Genokon Med Genokon Co, Xiamen 361115, Peoples R China
来源：
TOXINS | 2018年 / 10卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Aspergillus flavus; Rum1; conidiation; sclerotia; aflatoxin; DNA-BINDING PROTEINS; SCLEROTIAL PRODUCTION; SECONDARY METABOLISM; HISTONE DEMETHYLASES; GENE; NIDULANS; FINGER; FAMILY; ARABIDOPSIS; REVEALS;
D O I：
10.3390/toxins10070301
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Aspergillus flavus produces mycotoxins especially aflatoxin B-1 and infects crops worldwide. As a PHD transcription factor, there is no report on the role of Rum1 in the virulence of Aspergillus spp. yet. This study explored the biological function of Rum1 in A. flavus through the construction of rum1 deletion mutants and rum1 complementation strains with the method of homologous recombination. It was found, in the study, that Rum1 negatively regulates conidiation through abaA and brlA, positively regulates sclerotia formation through nsdC, nsdD, and sclR, triggers aflatoxin biological synthesis, and enhances the activity of amylase. Our findings suggested that Rum1 plays a major role in the growth of mycelia, conidia, and sclerotia production along with aflatoxin biosynthesis in A. flavus.
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页数：16
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