Reducing image distortions due to temperature-related microscope stage drift

被引：21

作者：

Adler, J ^{[1
]}

Pagakis, SN ^{[1
]}

机构：

[1] Natl Inst Med Res, Confocal & Image Anal Lab, London NW7 1AA, England

来源：

JOURNAL OF MICROSCOPY-OXFORD | 2003年 / 210卷

关键词：

air conditioning; confocal microscopy; image quality; microscope design; microscope stage movements; temperature control;

D O I：

10.1046/j.1365-2818.2003.01160.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Distortions in confocal 3D image data sets were related to movements of the microscope stage that matched fluctuations in laboratory temperature. Movement was apparent in all three axes. Adding a draught-proof enclosure, covering the stage, objective lenses and supporting structures, minimized these short-term fluctuations. However, the stage still tracked slow changes in laboratory temperature and was sensitive to heat sources mounted on the microscope. Suggestions are made about microscope design.

引用

页码：131 / 137

页数：7

共 11 条

[1] Optical far-field microscopy of single molecules with 3.4 nm lateral resolution [J].

Bloess, A ;

Durand, Y ;

Matsushita, M ;

Van der Meer, H ;

Brakenhoff, GJ ;

Schmidt, J .

JOURNAL OF MICROSCOPY-OXFORD, 2002, 205 :76-85

[2] Tissue shrinkage and unbiased stereological estimation of particle number and size [J].

Dorph-Petersen, KA ;

Nyengaard, JR ;

Gundersen, HJG .

JOURNAL OF MICROSCOPY-OXFORD, 2001, 204 :232-246

[3] Focal spots of size λ/23 open up far-field florescence microscopy at 33 nm axial resolution -: art. no. 163901 [J].

Dyba, M ;

Hell, SW .

PHYSICAL REVIEW LETTERS, 2002, 88 (16) :4-163901

[4] REDUCTION OF NOISE IN TV RATE ELECTRON-MICROSCOPE IMAGES BY DIGITAL FILTERING [J].

ERASMUS, SJ .

JOURNAL OF MICROSCOPY-OXFORD, 1982, 127 (JUL) :29-37

[5]

Periasamy A., 2001, METHODS CELLULAR IMA

[6] Single-particle tracking: Applications to membrane dynamics [J].

Saxton, MJ ;

Jacobson, K .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1997, 26 :373-399

[7] Simultaneous measurement of Ca2+ and cellular dynamics:: Combined scanning ion conductance and optical microscopy to study contracting cardiac myocytes [J].

Shevchuk, AI ;

Gorelik, J ;

Harding, SE ;

Lab, MJ ;

Klenerman, D ;

Korchev, YE .

BIOPHYSICAL JOURNAL, 2001, 81 (03) :1759-1764

[8] Measuring tubulin content in Toxoplasma gondii:: A comparison of laser-scanning confocal and wide-field fluorescence microscopy [J].

Swedlow, JR ;

Hu, K ;

Andrews, PD ;

Roos, DS ;

Murray, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (04) :2014-2019

[9]

TERASAKI M, 1995, HDB BIOL CONFOCAL MI, P339

[10]

Zucker RM, 2001, CYTOMETRY, V44, P273, DOI 10.1002/1097-0320(20010801)44:4<273::AID-CYTO1120>3.0.CO

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