Recent Advances in Rapid and Highly Sensitive Detection of Proteins and Specific DNA Sequences Using a Magnetic Modulation Biosensing System

被引：4

作者：

Roth, Shira ^{[1
]}

Margulis, Michael ^{[1
]}

Danielli, Amos ^{[1
]}

机构：

[1] Bar Ilan Univ, Inst Nanotechnol & Adv Mat, Fac Engn, Max & Anna Webb St, IL-5290002 Ramat Gan, Israel

来源：

SENSORS | 2022年 / 22卷 / 12期

基金：

以色列科学基金会;

关键词：

magnetic beads; biosensing; nucleic acids; serology; protein-protein interactions; magnetic modulation biosensing; SURFACE-PLASMON RESONANCE; ASSAY;

D O I：

10.3390/s22124497

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In early disease stages, biomolecules of interest exist in very low concentrations, presenting a significant challenge for analytical devices and methods. Here, we provide a comprehensive overview of an innovative optical biosensing technology, termed magnetic modulation biosensing (MMB), its biomedical applications, and its ongoing development. In MMB, magnetic beads are attached to fluorescently labeled target molecules. A controlled magnetic force aggregates the magnetic beads and transports them in and out of an excitation laser beam, generating a periodic fluorescent signal that is detected and demodulated. MMB applications include rapid and highly sensitive detection of specific nucleic acid sequences, antibodies, proteins, and protein interactions. Compared with other established analytical methodologies, MMB provides improved sensitivity, shorter processing time, and simpler protocols.

引用

页数：22

共 62 条

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