En Face and Cross-sectional Corneal Tomograms Using Sub-micron spatial resolution Optical Coherence Tomography

被引：13

作者：

Chen, Yu-Tung ^{[1
]}

Tsai, Chia-Ying ^{[2
,3
,4
]}

Chiu, Yu-Kuang ^{[1
]}

Hsu, Ting-Wei ^{[1
]}

Chen, Lily Wei ^{[2
]}

Chen, Wei-Li ^{[2
,5
]}

Huang, Sheng-Lung ^{[1
,6
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Photon & Optoelect, Taipei, Taiwan

[2] Natl Taiwan Univ Hosp, Dept Ophthalmol, Taipei, Taiwan

[3] Fu Jen Catholic Univ Hosp, Dept Ophthalmol, New Taipei, Taiwan

[4] Fu Jen Catholic Univ, Coll Med, Sch Med, New Taipei, Taiwan

[5] Natl Taiwan Univ, Coll Med, Dept Ophthalmol, Taipei, Taiwan

[6] Natl Taiwan Univ, Dept Elect Engn, Taipei, Taiwan

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

ENDOTHELIUM; MICROSCOPY; LAYER;

D O I：

10.1038/s41598-018-32814-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Accurate diagnosis of corneal pathology and morphological identification of different corneal layers require clear delineation of corneal three-dimensional structures and en face or cross-sectional imaging of palisade of Vogt (POV), neovascularization (NV) or corneal nerves. Here we report a prototype of full-field optical coherence tomography (FF-OCT) system with isotropic sub-micron spatial resolution in the en face and cross-sectional views. It can also provide three-dimensional reconstructed images and a large field of view (FOV) by stitching tomograms side by side. We validated the imaging power of this prototype in in vivo rat and rabbit eyes, and quantified anatomical characteristics such as corneal layer thickness, endothelial cell density and the intensity profile of different layers. This FF-OCT delineated the ridge-like structure of POV, corneal nerve bundles, and conjunctival vessels in rat eyes. It also clearly identified the vessel walls and red blood cells in rabbit model of corneal NV. The findings provided by this FF-OCT are expected to facilitate corneal disease diagnosis and treatment.

引用

页数：10

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