Reversible Positioning of Single Molecules inside Zero-Mode Waveguides

被引：50
|
作者：
Larkin, Joseph [1 ,2 ]
Foquet, Mathieu [3 ]
Turner, Stephen W. [3 ]
Korlach, Jonas [3 ]
Wanunu, Meni [1 ,2 ]
机构：
[1] Northeastern Univ, Dept Phys, Boston, MA 02115 USA
[2] Northeastern Univ, Dept Chem Chem Biol, Boston, MA 02115 USA
[3] Pacific Biosci, Menlo Pk, CA 94025 USA
来源：
NANO LETTERS | 2014年 / 14卷 / 10期
基金：
美国国家科学基金会;
关键词：
SMRT-sequencing; DNA sequencing; single molecule; nanophotonics; zeptoliter; DNA; NANOPORE; SPECTROSCOPY; RECOGNITION; DEPOSITION;
D O I：
10.1021/nl503134x
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of magnitude higher than diffusion-based molecular loading.
引用
收藏
页码：6023 / 6029
页数：7
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