Selection of reference genes for gene expression studies in heart failure for left and right ventricles

被引：18
作者：
Li, Mengmeng
Rao, Man
Chen, Kai
Zhou, Jianye
Song, Jiangping [1 ,2 ]
机构：
[1] Chinese Acad Med Sci, Natl Ctr Cardiovasc Dis, Fuwai Hosp, State Key Lab Cardiovasc Dis, 167A Beilishi Rd, Beijing 100037, Peoples R China
[2] Peking Union Med Coll, 167A Beilishi Rd, Beijing 100037, Peoples R China
来源：
GENE | 2017年 / 620卷
基金：
中国国家自然科学基金;
关键词：
Heart failure; Housekeeping gene; Right and left ventricles; qRT-PCR; MESSENGER-RNA EXPRESSION; QUANTITATIVE RT-PCR; REAL-TIME PCR; HOUSEKEEPING GENES; DILATED CARDIOMYOPATHY; MYOCARDIAL-ISCHEMIA; NORMALIZATION; MODEL; MICROARRAY; HYPOXIA;
D O I：
10.1016/j.gene.2017.04.006
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Real-time quantitative reverse transcriptase-PCR (qRT-PCR) is a feasible tool for determining gene expression profiles, but the accuracy and reliability of the results depends on the stable expression of selected housekeeping genes in different samples. By far, researches on stable housekeeping genes in human heart failure samples are rare. Moreover the effect of heart failure on the expression of housekeeping genes in right and left ventricles is yet to be studied. Therefore we aim to provide stable housekeeping genes for both ventricles in heart failure and normal heart samples. In this study, we selected seven commonly used housekeeping genes as candidates. By using the qRT-PCR, the expression levels of ACTB, RAB7A, GAPDH, REEP5, RPL5, PSMB4 and VCP in eight heart failure and four normal heart samples were assessed. The stability of candidate housekeeping genes was evaluated by geNorm and Normfinder softwares. GAPDH showed the least variation in all heart samples. Results also indicated the difference of gene expression existed in heart failure left and right ventricles. GAPDH had the highest expression stability in both heart failure and normal heart samples. We also propose using different sets of housekeeping genes for left and right ventricles respectively. The combination of RPL5, GAPDH and PSMB4 is suitable for the right ventricle and the combination of GAPDH, REEP5 and RAB7A is suitable for the left ventricle.
引用
收藏
页码：30 / 35
页数：6
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