Surface modification of titanium with hydroxyapatite layer induced by phase-transited lysozyme coating

被引：30

作者：

Yang, Jie ^{[1
]}

Zhang, Kai ^{[2
]}

Que, Kehua ^{[1
]}

Hou, Shuai ^{[3
]}

Chen, Zhen ^{[1
]}

Li, Ying ^{[1
]}

Wang, Yao ^{[1
]}

Song, Yunjia ^{[1
]}

Guan, Binbin ^{[3
]}

Zhang, Wenxin ^{[1
]}

Zhu, Dongwang ^{[1
]}

Li, Changyi ^{[1
]}

Wang, Dong ^{[3
]}

Geng, Peng ^{[2
]}

Zhang, Xu ^{[1
]}

机构：

[1] Tianjin Med Univ, Sch & Hosp Stomatol, 12 Observ Rd, Tianjin 30070, Peoples R China

[2] Tianjin Med Univ, Sch Basic Med Sci, Dept Pathogen Biol, 12 Observ Rd, Tianjin 30070, Peoples R China

[3] Tianjin Med Univ, Gen Hosp, Dept Stomaool, 154 Anshan Rd, Tianjin 300052, Peoples R China

来源：

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2018年 / 92卷

基金：

中国国家自然科学基金;

关键词：

OSTEOGENIC DIFFERENTIATION; STROMAL CELLS; BONE; APATITE; OSTEOINDUCTIVITY; PROLIFERATION; PHOSPHATE; SUBSTRATE; BEHAVIOR;

D O I：

10.1016/j.msec.2018.05.055

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Surface modification of titanium with a hydroxyapatite (HAP) coating can improve the bioactivity of pristine titanium. The traditional techniques for coating HAP on titanium involve nonmild treatments using strong bases or acids or high temperatures. In this study, the coating of HAP was carried out by a novel methodology called phase-transited lysozyme-assisted hydroxyapatite formation (PAH); in this process of biomimetic mineralization, the abundant functional carboxyl groups of phase-transited lysozyme (PTL) were responsible for the nucleation of HAP crystals by concentrating Ca2+ ions at the interface between PTL and CaCl2 solution and for the subsequent growth of HAP crystals occurring in simulated body fluid (SBF). In vitro and in vivo experiments verified that the surface of titanium modified with the HAP/PTL-Ti multilayer was endowed with improved bio-compatibility and osteoinductivity compared with those of pristine titanium. Therefore, PAH is a simple, rapid, low-cost and green process for the surface modification of titanium with an HAP coating and thus will be a promising methodology for the surface modification of titanium implants.

引用

页码：206 / 215

页数：10