Localization of two forms of phospholipase C-β1, a and b, in C6Bu-1 cells

被引：72
作者：
Bahk, YY
Song, H
Baek, SH
Park, BY
Kim, H
Ryu, SH
Suh, PG
机构：
[1] Pohang Univ Sci & Technol, Dept Life Sci, Pohang 790784, Kyungbuk, South Korea
[2] Korea Univ, Coll Med, Dept Anat, Seoul 136701, South Korea
[3] Chonnam Univ, Coll Dent, Dept Biochem, Kwong Yu, South Korea
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1998年 / 1389卷 / 01期
关键词：
phospholipase C-beta 1; C6Bu-1; cell; immunocytochemistry; nuclear localization;
D O I：
10.1016/S0005-2760(97)00128-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phospholipase C-beta 1 (PLC-beta 1), one of the PLC-beta isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-beta 1a) and 140 kDa (PLC-beta 1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene, In this study, the subcellular localization of the two phospholipases C-beta 1 proteins was examined in rat CGBu-1 glioma cells using immunological techniques, Immunoblot analysis revealed that the two forms of PLC-beta 1 were detectible in both cytosolic and nuclear fractions, PLC-beta 1a appeared to be located preferentially in the cytosol, whereas PLC-beta 1b was found predominantly in the nuclei of C6Bu-1 cells, Immunocytochemical experiments confirmed the differential localization of the two PLC-beta 1 species in C6Bu-1 cells. These results suggest that the two PLC-beta 1 proteins may have different physiological roles in the cell. (C) 1998 Elsevier Science B.V.
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页码：76 / 80
页数：5
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