Genomic instability in non-neoplastic oral mucosa cells can predict risk during 4-nitroquinoline 1-oxide-induced rat tongue carcinogenesis

被引：52

作者：

Ribeiro, DA ^{[1
]}

Salvadori, DMF ^{[1
]}

da Silva, RN ^{[1
]}

Darros, BR ^{[1
]}

Marques, MEA ^{[1
]}

机构：

[1] Univ Estadual Paulista, Fac Med Botucatu, TOXICAN, Dept Patol Nucl Avaliacao Toxicogenet & Cancerige, BR-18618000 Sao Paulo, Brazil

来源：

ORAL ONCOLOGY | 2004年 / 40卷 / 09期

基金：

巴西圣保罗研究基金会;

关键词：

single cell gel (comet) assay; oral cancer; 4-nitroquinoline; 1-oxide;

D O I：

10.1016/j.oraloncology.2004.04.010

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

4-Nitroquinotine 1-oxide (4NQO)-induced rat tongue carcinogenesis is a useful model for studying oral squamous cell carcinoma. The aim of this study was to investigate the level of DNA damage induced by 4NQO in oral mucosa cells by the single cell get (comet) assay. Mate Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution by drinking water for 4, 12 or 20 weeks. Ten animals were used as negative control. Statistically significant increase of DNA damage was observed in non-neoplastic oral cells at four weeks of 4NQO administration when compared with control (P < 0.05). The level of DNA damage was directly associated with the severity of histological changes. The results suggest that histologically normal tissue is able to harbor genetically unstable cells contributing to the initiation of oral carcinogenesis. Genomic instability appears to be associated with the risk and progression of oral cancer. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：910 / 915

页数：6

共 24 条

[1]

Califano J, 1996, CANCER RES, V56, P2488

[2] DNA damage in cytologically normal urothelial cells of patients with a history of urothelial cell carcinoma [J].

Gontijo, AMD ;

Marcondes, JPD ;

Elias, FN ;

de Oliveira, MLCS ;

de Lima, ROA ;

Salvadori, DMF ;

de Camargo, JLV .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2002, 40 (03) :190-199

[3]

Gontijo AMDC, 2001, CANCER EPIDEM BIOMAR, V10, P987

[4] Genetic polymorphisms of drug-metabolizing enzymes and susceptibility to oral cavity cancer [J].

Hahn, M ;

Hagedorn, G ;

Kuhlisch, E ;

Schackert, HK ;

Eckelt, U .

ORAL ONCOLOGY, 2002, 38 (05) :486-490

[5] Recommendations for conducting the in vivo alkaline Comet assay [J].

Hartmann, A ;

Agurell, E ;

Beevers, C ;

Brendler-Schwaab, S ;

Burlinson, B ;

Clay, P ;

Collins, A ;

Smith, A ;

Speit, G ;

Thybaud, V ;

Tice, RR .

MUTAGENESIS, 2003, 18 (01) :45-51

[6] CELL-CYCLE CONTROL AND CANCER [J].

HARTWELL, LH ;

KASTAN, MB .

SCIENCE, 1994, 266 (5192) :1821-1828

[7] The contribution of endogenous sources of DNA damage to the multiple mutations in cancer [J].

Jackson, AL ;

Loeb, LA .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2001, 477 (1-2) :7-21

[8]

Limoli CL, 1997, CANCER RES, V57, P4048

[9] CANCER IN DEVELOPING-COUNTRIES - OPPORTUNITY AND CHALLENGE [J].

MAGRATH, I ;

LITVAK, J .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1993, 85 (11) :862-874

[10] Changes in Bcl-2 and Bax expression in rat tongue during 4-nitroquinoline 1-oxide-induced carcinogenesis [J].

Nishimura, A .

JOURNAL OF DENTAL RESEARCH, 1999, 78 (06) :1264-1269

← 1 2 3 →