Molecular cloning and functional analysis of the zebrafish follicle-stimulating hormone (FSH)β promoter

被引：8
作者：
Chen, Jyh-Yih [1 ]
Chiou, Ming-Jyun [1 ]
Chen, Lee-Kang [1 ]
Wu, Jen-Leih [2 ]
机构：
[1] Acad Sinica, Inst Cellular & Organism Biol, Marine Res Stn, Jiaushi 262, Ilan, Taiwan
[2] Acad Sinica, Inst Cellular & Organism Biol, Taipei 115, Taiwan
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2010年 / 155卷 / 02期
关键词：
Follicle-stimulating hormone gene; Promoter analysis; Zebrafish; GONADOTROPIN-RELEASING-HORMONE; PROTEIN-KINASE-C; SUBUNIT GENES; GNRH RECEPTOR; BETA GENE; TRANSGENIC MICE; GRANULOSA-CELLS; EXPRESSION; FSH; PITUITARY;
D O I：
10.1016/j.cbpb.2009.10.017
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the present study, we cloned and characterized a zebrafish follicle-stimulating hormone (zfFSH)beta promoter with deletion fragments transfected into a tilapia ovary (TO2) cell line, and demonstrated that the zfFSH beta promoter responded to 6 h of gonadotropin-releasing hormone (GnRH) treatment by activating calcium influx and protein kinase C (PKC), but after 24 h, GnRH induction was generated by activation of extracellular-regulated kinase (ERK)1/2 and repression by PKC. Furthermore, to study the promoter-specific expression, we constructed a series of FSH beta (4.0-, 3.0-, 2.0-, and 1.0-kb) promoter-driven green fluorescent protein (GFP) fragments encoding the GFP complementary DNA transgene which was microinjected into zebrafish embryos. Morphological studies of transgenic zebrafish indicated that the FSH beta promoter-driven GFP transcripts appeared in the heart, skin, and vertebrae. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：155 / 163
页数：9
相关论文
共 35 条
[1]   Tilapia follicle-stimulating hormone (FSH): Immunochemistry, stimulation by gonadotropin-releasing hormone, and effect of biologically active recombinant FSH on steroid secretion [J].
Aizen, Joseph ;
Kasuto, Harel ;
Golan, Matan ;
Zakay, Hila ;
Levavi-Sivan, Berta .
BIOLOGY OF REPRODUCTION, 2007, 76 (04) :692-700
[2]   REGULATION OF GONADOTROPIN MESSENGER-RNA LEVELS IN CULTURED RAT PITUITARY-CELLS BY GONADOTROPIN-RELEASING-HORMONE (GNRH) - ROLE FOR CA2+ AND PROTEIN-KINASE-C [J].
BENMENAHEM, D ;
NAOR, Z .
BIOCHEMISTRY, 1994, 33 (12) :3698-3704
[3]   Fetal expression of GnRH and GnRH receptor genes in rat testis and ovary [J].
Botté, MC ;
Chamagne, AM ;
Carré, MC ;
Counis, R ;
Kottler, ML .
JOURNAL OF ENDOCRINOLOGY, 1998, 159 (01) :179-189
[4]   Poor ovarian response to gonadotropin stimulation is associated with low expression of follicle-stimulating hormone receptor in granulosa cells [J].
Cai, Jie ;
Lou, Hang-ying ;
Dong, Min-yue ;
Lu, Xiu-e ;
Zhu, Yi-min ;
Gao, Hui-juan ;
Huang, He-feng .
FERTILITY AND STERILITY, 2007, 87 (06) :1350-1356
[5]   Cloning and expression analysis of a protein kinase C gene, PKCμ, and its regulation of the promoter region in Zebrafish [J].
Chen, Jyh-Yih ;
Wei, Chuan-Chih ;
Chiou, Ming-Jyun ;
Su, Hung-Yi ;
Kuo, Ching-Ming .
DNA AND CELL BIOLOGY, 2007, 26 (06) :415-424
[6]   Activation of Mitogen-activated protein kinase (MAPK) by GnRH is cell-context dependent [J].
Dobkin-Bekman, Masha ;
Naidich, Michal ;
Pawson, Adam J. ;
Millar, Robert P. ;
Seger, Rony ;
Naor, Zvi .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2006, 252 (1-2) :184-190
[7]   High-efficiency gene transfection of macrophages by lipoplexes [J].
Dokka, S ;
Toledo, D ;
Shi, XL ;
Ye, JP ;
Rojanasakul, Y .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2000, 206 (1-2) :97-104
[8]   Intrafollicular paracrine communication in the zebrafish ovary: The state of the art of an emerging model for the study of vertebrate folliculogenesis [J].
Ge, W .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2005, 237 (1-2) :1-10
[9]   PARTIAL CHARACTERIZATION OF THE GONADOTROPIN-RELEASING-HORMONE (GNRH) GENE TRANSCRIPT IN THE RAT OVARY [J].
GOUBAU, S ;
BOND, CT ;
ADELMAN, JP ;
MISRA, V ;
HYNES, MF ;
SCHULTZ, GA ;
MURPHY, BD .
ENDOCRINOLOGY, 1992, 130 (05) :3098-3100
[10]   GnRH signaling pathways regulate differentially the tilapia gonadotropin subunit genes [J].
Gur, G ;
Bonfil, D ;
Safarian, H ;
Naor, Z ;
Yaron, Z .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2002, 189 (1-2) :125-134
1234