A Toolbox of Fluorescent Peptide Biosensors to Highlight Protein Kinases in Complex Samples: Focus on Cyclin-Dependent Kinases

被引:3
作者
Morris, May C. [1 ]
机构
[1] Univ Montpellier, Pole Chim Balard Rech, Inst Biomol Max Mousseron, 1919 Route Mende, F-34293 Montpellier, France
关键词
Biosensors; Cancer; Fluorescence; Kinase; Peptides; CELL-PENETRATING PEPTIDES; LIVE-CELL; MOLECULAR-MECHANISMS; SENSOR PROTEINS; RECENT PROGRESS; GENERAL-METHOD; IN-VITRO; DESIGN; PROBES; PHOSPHORYLATION;
D O I
10.1002/ejoc.202200120
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
Development of sensitive and selective strategies for detection of disease biomarkers constitutes a major challenge for diagnostics and companion assays. New strategies are required to advance personalized and point-of-care medicine. Protein kinases (PK) are hyperactivated in many human cancers, therefore constituting relevant biomarkers and attractive pharmacological targets.Yet quantification of PK expression levels alone does not convey an accurate readout of their activity, since PKs are subject to numerous interactions and post-translational regulations. Hence technologies that report on PK function are essential for complete appreciation of their behaviour and dysregulation in physio-pathological settings. This Review focuses on fluorescent peptide biosensors designed to report on PK activities, and in particular on a toolbox of biosensors tailored to monitor cyclin-dependent kinases. These selective optical probes offer a straightforward means of quantifying differences in kinase activities between healthy and cancer cells, and can report on alterations in response to therapeutics in vitro, in living cells and in vivo.
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页数:8
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共 80 条
  • [1] Anai T., 2007, J AM CHEM SOC, V132, P5619
  • [2] The history and future of targeting cyclin-dependent kinases in cancer therapy
    Asghar, Uzma
    Witkiewicz, Agnieszka K.
    Turner, Nicholas C.
    Knudsen, Erik S.
    [J]. NATURE REVIEWS DRUG DISCOVERY, 2015, 14 (02) : 130 - 146
  • [3] Silafluorene as a promising core for cell-permeant, highly bright and two-photon excitable fluorescent probes for live-cell imaging
    Auvray, Marie
    Bolze, Frederic
    Clavier, Gilles
    Mahuteau-Betzer, Florence
    [J]. DYES AND PIGMENTS, 2021, 187 (187)
  • [4] Design of a protein kinase-inducible domain
    Balakrishnan, S
    Zondlo, NJ
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (17) : 5590 - 5591
  • [5] Semisynthetic Fluorescent Sensor Proteins Based on Self-Labeling Protein Tags
    Brun, Matthias A.
    Tan, Kui-Thong
    Nakata, Eiji
    Hinner, Marlon J.
    Johnsson, Kai
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (16) : 5873 - 5884
  • [6] Biosensors of protein kinase action: from in vitro assays to living cells
    Chen, CA
    Yell, RH
    Yan, XW
    Lawrence, DS
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2004, 1697 (1-2): : 39 - 51
  • [7] Kinase drug discovery 20 years after imatinib
    Cohen, Philip
    Cross, Darren
    Janne, Pasi A.
    [J]. NATURE REVIEWS DRUG DISCOVERY, 2022,
  • [8] Fluorescence Lifetime Imaging of Biosensor Peptide Phosphorylation in Single Live Cells
    Damayanti, Nur P.
    Parker, Laurie L.
    Irudayaraj, Joseph M. K.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (14) : 3931 - 3934
  • [9] Vinyl-triphenylamine dyes, a new family of switchable fluorescent probes for targeted two-photon cellular imaging: from DNA to protein labeling
    Dumat, Blaise
    Bordeau, Guillaume
    Aranda, Ana I.
    Mahuteau-Betzer, Florence
    El Harfouch, Yara
    Metge, Germain
    Charra, Fabrice
    Fiorini-Debuisschert, Celine
    Teulade-Fichou, Marie-Paule
    [J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2012, 10 (30) : 6054 - 6061
  • [10] Ten things you should know about protein kinases: IUPHAR Review 14
    Fabbro, Doriano
    Cowan-Jacob, Sandra W.
    Moebitz, Henrik
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 2015, 172 (11) : 2675 - 2700