Identifying Bird Remains Using Ancient DNA Barcoding

被引:13
作者
Dalen, Love [1 ]
Lagerholm, Vendela K. [1 ,2 ,3 ]
Nylander, Johan A. A. [1 ]
Barton, Nick [4 ]
Bochenski, Zbigniew M. [5 ]
Tomek, Teresa [5 ]
Rudling, David [6 ]
Ericson, Per G. P. [1 ]
Irestedt, Martin [1 ]
Stewart, John R. [7 ]
机构
[1] Swedish Museum Nat Hist, Dept Bioinformat & Genet, Box 50007, SE-10405 Stockholm, Sweden
[2] Stockholm Univ, Dept Zool, SE-10691 Stockholm, Sweden
[3] Univ St Andrews, Sch Geog & Sustainable Dev, North St, St Andrews KY16 9AL, Fife, Scotland
[4] Inst Archaeol, 36 Beaumont St, Oxford OX1 2PG, England
[5] Polish Acad Sci, Inst Systemat & Evolut Anim, Slawkowska 17, PL-31016 Krakow, Poland
[6] Sussex Sch Archaeol, Mays Farm, Polegate BN26 6TS, Sussex, England
[7] Bournemouth Univ, Fac Sci & Technol, Talbot Campus, Poole BH12 5BB, Dorset, England
基金
瑞典研究理事会;
关键词
Aves; species identification; 16S; palaeogenetics; biodiversity; archaeology; palaeontology; NEW-ZEALAND; IDENTIFICATION; SAMPLES; CAVE;
D O I
10.3390/genes8060169
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Bird remains that are difficult to identify taxonomically using morphological methods, are common in the palaeontological record. Other types of challenging avian material include artefacts and food items from endangered taxa, as well as remains from aircraft strikes. We here present a DNA-based method that enables taxonomic identification of bird remains, even from material where the DNA is heavily degraded. The method is based on the amplification and sequencing of two short variable parts of the 16S region in the mitochondrial genome. To demonstrate the applicability of this approach, we evaluated the method on a set of Holocene and Late Pleistocene postcranial bird bones from several palaeontological and archaeological sites in Europe with good success.
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页数:8
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