Effect of vasoactive intestinal peptide on pulmonary surfactants phospholipid synthesis in lung explants

被引：0

作者：

Li, L ^{[1
]}

Luo, ZQ ^{[1
]}

Zhou, FW ^{[1
]}

Feng, DD ^{[1
]}

Guan, CX ^{[1
]}

Zhang, CQ ^{[1
]}

Sun, XH ^{[1
]}

机构：

[1] Cent S Univ, Xiangya Sch Med, Dept Physiol, Changsha 410078, Peoples R China

来源：

ACTA PHARMACOLOGICA SINICA | 2004年 / 25卷 / 12期

关键词：

vasoactive intestinal peptide; pulmonary surfactants; choline-phosphate cytidylyltransferase;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To investigate the effect of vasoactive intestinal peptide (VIP) on pulmonary surfactants (PS) phospholipid synthesis in cultured lung explants. METHODS: Lung explants were cultured with serum-free medium, [methyl-H-3]choline incorporation, total phospholipid, phosphatidylcholine, activity of choline-phosphate cytidylyltransferase (CCT) and CCTalpha mRNA level in lung explants were determined. RESULTS: (1) VIP (10(-10)-10(-7) mol/L) for 16 h promoted [methyl-H-3]choline incorporation in dose dependence and VIP (10(-8) mol/L) for 2 h-16 h promoted [methyl-H-3]choline incorporation in time dependence. (2) VIP (10(-8) mol/L) enhanced the contents of total phospholipids and phosphatidylcholine in lung explants. (3) VIP (10(-10)-10(-7) mol/L) elevated microsomal CCT activity of lung explants in dose dependence. (4) VIP (10(-8) mol/L) increased expression of CCTalpha mRNA in lung explants and alveolar type II cells (ATII). (5) [D-P-Cl-Phe(6)-Leu(17)]-VIP (10(-6) mol/L), a VIP receptors antagonist, abolished the increase of [H-3]choline incorporation, microsomal CCT activity and CCTalpha mRNA level induced by VIP (10(-8) mol/L) in lung explants. CONCLUSION: VIP could enhance synthesis of phosphatidylcholine, the major component of pulmonary surfactants by enhancing microsomal CCT activity and CCTalpha mRNA level via VIP receptor-mediated pathway.

引用

页码：1652 / 1658

页数：7

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