Separation of profen enantiomers by capillary electrophoresis using cyclodextrins as chiral selectors

被引：63

作者：

Blanco, M ^{[1
]}

Coello, J ^{[1
]}

Iturriaga, H ^{[1
]}

Maspoch, S ^{[1
]}

Pérez-Maseda, C ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Fac Ciencias, Dept Quim, Unitat Quim Analit, E-08193 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 793卷 / 01期

关键词：

enantiomeric separation; pharmaceutical analysis; buffer composition; arylpropionic acids; ketoprofen; ibuprofen; fenoprofen; profens;

D O I：

10.1016/S0021-9673(97)00893-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for resolving the enantiomers of various 2-arylpropionic acids (viz. keroprofen, ibuprofen and fenoprofen) by capillary zone electrophoresis (CZE) using a background electrolyte (BGE) containing a cyclodextrin as chiral selector is proposed. The effects of the type of cyclodextrin used and its concentration on resolution were studied and heptakis-2,3,6-tri-O-methyl-beta-cyclodextrin was found to be the sole effective choice for the quantitative enantiomeric resolution of all the compounds tested, The influence of pH, BGE concentration, capillary temperature and addition of methanol to the BGE on resolution and other separation-related parameters was also studied. The three compounds studied can be enantiomerically resolved with a high efficiency in a short time (less than 20 min) with no capillary treatment. This makes the proposed method suitable for assessing the enantiomeric purity of commercially available pharmaceuticals. (C) 1998 Elsevier Science B.V.

引用

页码：165 / 175

页数：11

共 28 条

[1]

ALLEMARK SG, 1988, CHROMATOGRAPHIC ENAN

[2] CHIRAL SEPARATION OF BASIC DRUGS BY CAPILLARY ZONE ELECTROPHORESIS WITH CYCLODEXTRIN ADDITIVES [J].

BECHET, I ;

PAQUES, P ;

FILLET, M ;

HUBERT, P ;

CROMMEN, J .

ELECTROPHORESIS, 1994, 15 (06) :818-823

[3] NEUTRAL AND ANIONIC CYCLODEXTRINS IN CAPILLARY ZONE ELECTROPHORESIS - ENANTIOMERIC SEPARATION OF EPHEDRINE AND RELATED-COMPOUNDS [J].

DETTE, C ;

EBEL, S ;

TERABE, S .

ELECTROPHORESIS, 1994, 15 (06) :799-803

[4] QUANTITATION IN CHIRAL CAPILLARY ELECTROPHORESIS - THEORETICAL AND PRACTICAL CONSIDERATIONS [J].

DHULST, A ;

VERBEKE, N .

ELECTROPHORESIS, 1994, 15 (06) :854-863

[5] CHIRAL SEPARATION BY CAPILLARY ELECTROPHORESIS WITH OLIGOSACCHARIDES [J].

DHULST, A ;

VERBEKE, N .

JOURNAL OF CHROMATOGRAPHY, 1992, 608 (1-2) :275-287

[6] Chiral discrimination by modified cyclodextrins [J].

Easton, CJ ;

Lincoln, SF .

CHEMICAL SOCIETY REVIEWS, 1996, 25 (03) :163-+

[7] ENANTIOMER RESOLUTION BY USING CAPILLARY ZONE ELECTROPHORESIS - RESOLUTION OF RACEMIC TRYPTOPHAN AND DETERMINATION OF THE ENANTIOMER COMPOSITION OF COMMERCIAL PHARMACEUTICAL EPINEPHRINE [J].

FANALI, S ;

BOCEK, P .

ELECTROPHORESIS, 1990, 11 (09) :757-760

[8] USE OF CYCLODEXTRINS FOR THE ENANTIOSELECTIVE SEPARATION OF ERGOT ALKALOIDS BY CAPILLARY ZONE ELECTROPHORESIS [J].

FANALI, S ;

FLIEGER, M ;

STEINEROVA, N ;

NARDI, A .

ELECTROPHORESIS, 1992, 13 (1-2) :39-43

[9] USE OF CYCLODEXTRINS IN CAPILLARY ELECTROPHORESIS FOR THE CHIRAL RESOLUTION OF SOME 2-ARYLPROPIONIC ACID NONSTEROIDAL ANTIINFLAMMATORY DRUGS [J].

FANALI, S ;

ATURKI, Z .

JOURNAL OF CHROMATOGRAPHY A, 1995, 694 (01) :297-305

[10] Comparison and modeling study of vancomycin, ristocetin A, and teicoplanin for CE enantioseparations [J].

Gasper, MP ;

Berthod, A ;

Nair, UB ;

Armstrong, DW .

ANALYTICAL CHEMISTRY, 1996, 68 (15) :2501-2514

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