Organization of the interferon-inducible 2′,5′-oligoadenylate-dependent ribonuclease L (RNase L) gene of mouse

被引：2
|
作者：
Pandey, Mitali [1 ]
Rath, Pramod C. [1 ]
机构：
[1] Jawaharlal Nehru Univ, Sch Life Sci, Mol Biol Lab, New Delhi 110067, India
来源：
MOLECULAR BIOLOGY REPORTS | 2007年 / 34卷 / 02期
关键词：
antiviral response; interferon; murine RNase L; gene structure; 5'-flanking DNA;
D O I：
10.1007/s11033-006-9020-z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Interferons (IFNs) induce a 2',5'-oligoadenylate (2-5A)-dependent ribonuclease L (RNase L) following virus-infection of mammalian cells. RNase L degrades both viral and cellular RNAs and restricts virus-proliferation. We have studied organization of RNase L gene in genomic DNA from the mouse liver by Southern blot analysis. Several BamHI, BglII, EcoRI, HincII, HindIII, NcoI, PstI, SacI, and XbaI restriction fragments hybridized to P-32-labeled mouse RNase L cDNA and the 5'-proximal exon probes. Mouse RNase L gene exists as a single copy (> 16 kb DNA) gene. A 5 kb HindIII and a 2.5 kb EcoRI DNA were detected as 5'-upstream DNA of the gene which may contain mouse RNase L promoter. Our results will help studying mouse RNase L gene promoter in further details.
引用
收藏
页码：97 / 104
页数：8
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