Isolation and Comparative Genomic Analysis of T1-Like Shigella Bacteriophage pSf-2

被引：19
作者：
Jun, Jin Woo [1 ,2 ]
Kim, Hyoun Joong [1 ,2 ]
Yun, Sae Kil [1 ,2 ]
Chai, Ji Young [3 ]
Lee, Byeong Chun [1 ,2 ]
Park, Se Chang [1 ,2 ]
机构：
[1] Seoul Natl Univ, Coll Vet Med, Seoul 151742, South Korea
[2] Seoul Natl Univ, Res Inst Vet Sci, Seoul 151742, South Korea
[3] Bundang Jesaeng Hosp, Dept Rheumatol, Songnam 463774, South Korea
来源：
CURRENT MICROBIOLOGY | 2016年 / 72卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
VIBRIO-PARAHAEMOLYTICUS; SEQUENCE; MULTICENTER; THERAPY; BURDEN; PHAGES;
D O I：
10.1007/s00284-015-0935-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The increasing prevalence of antibiotic-resistant Shigella sp. emphasizes that alternatives to conventional antibiotics are needed. Siphoviridae bacteriophage (phage), pSf-2, infecting S. flexneri ATCC (R) 12022 was isolated from Geolpocheon stream in Korea. Morphological analysis by transmission electron microscopy revealed that pSf-2 has a head of about 57 +/- 4 nm in diameter with a long tail of 136 +/- 3 nm in length and 15 +/- 2 nm in width. One-step growth analysis revealed that pSf-2 has latent period of 30 min and burst size of 16 PFU/infected cell. The DNA genome of pSf-2 is composed of 50,109 bp with a G+C content of 45.44 %. The genome encodes 83 putative ORFs, 19 putative promoters, and 23 transcriptional terminator regions. Genome sequence analysis of pSf-2 and comparative analysis with the homologous T1-like Shigella phages, Shfl1 and pSf-1, revealed that pSf-2 is a novel T1-like Shigella phage. These results showed that pSf-2 might have a high potential as a biocontrol agent to control shigellosis. Also, the genomic information may lead to further understanding of phage biodiversity, especially T1-like phages.
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收藏
页码：235 / 241
页数：7
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