Isolation and Comparative Genomic Analysis of T1-Like Shigella Bacteriophage pSf-2

被引：19

作者：

Jun, Jin Woo ^{[1
,2
]}

Kim, Hyoun Joong ^{[1
,2
]}

Yun, Sae Kil ^{[1
,2
]}

Chai, Ji Young ^{[3
]}

Lee, Byeong Chun ^{[1
,2
]}

Park, Se Chang ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Coll Vet Med, Seoul 151742, South Korea

[2] Seoul Natl Univ, Res Inst Vet Sci, Seoul 151742, South Korea

[3] Bundang Jesaeng Hosp, Dept Rheumatol, Songnam 463774, South Korea

来源：

CURRENT MICROBIOLOGY | 2016年 / 72卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

VIBRIO-PARAHAEMOLYTICUS; SEQUENCE; MULTICENTER; THERAPY; BURDEN; PHAGES;

D O I：

10.1007/s00284-015-0935-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The increasing prevalence of antibiotic-resistant Shigella sp. emphasizes that alternatives to conventional antibiotics are needed. Siphoviridae bacteriophage (phage), pSf-2, infecting S. flexneri ATCC (R) 12022 was isolated from Geolpocheon stream in Korea. Morphological analysis by transmission electron microscopy revealed that pSf-2 has a head of about 57 +/- 4 nm in diameter with a long tail of 136 +/- 3 nm in length and 15 +/- 2 nm in width. One-step growth analysis revealed that pSf-2 has latent period of 30 min and burst size of 16 PFU/infected cell. The DNA genome of pSf-2 is composed of 50,109 bp with a G+C content of 45.44 %. The genome encodes 83 putative ORFs, 19 putative promoters, and 23 transcriptional terminator regions. Genome sequence analysis of pSf-2 and comparative analysis with the homologous T1-like Shigella phages, Shfl1 and pSf-1, revealed that pSf-2 is a novel T1-like Shigella phage. These results showed that pSf-2 might have a high potential as a biocontrol agent to control shigellosis. Also, the genomic information may lead to further understanding of phage biodiversity, especially T1-like phages.

引用

页码：235 / 241

页数：7

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