Reagentless amperometric immunosensor for alpha-fetoprotein based on gold Nanoparticles/TiO2 colloids/prussian blue modified platinum electrode

被引：34

作者：

Yuan, Yanru ^{[1
]}

Ynan, Ruo ^{[1
]}

Chai, Yaqin ^{[1
]}

Zhuo, Ying ^{[1
]}

Shi, Yintao ^{[1
]}

He, Xildan ^{[1
]}

Miao, Xiangmin ^{[1
]}

机构：

[1] SW Univ, Coll Chem & Chem Engn, Chongqing Key Lab Analyt Chem, Chongqing 400715, Peoples R China

来源：

ELECTROANALYSIS | 2007年 / 19卷 / 13期

关键词：

alpha-fetoprotein (AFP); amperometric immunosensor; gold nanoparticles; prussian blue; TiO2; colloids;

D O I：

10.1002/elan.200603868

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel reagentless amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was prepared by immobilizing TiO2 colloids on Prussian blue (PB) modified platinum electrode, which yielded a positively charged interface with strong adsorption to deposit gold nanoparticles for immobilization of alpha-fetoprotein antibody (anti-AFP). The factors influencing the performance of the proposed immunosensors were studied in detail. Under the optimized conditions, cyclic voltammograms determination of AFP showed a specific response in two concentration ranges from 3.0 to 30.0 ng/mL and from 30.0 to 300.0 ng/mL with a detection limit of 1.0 ng/mL at a signal-to-noise ratio of 3. The proposed immunosensor exhibited high selectivity, good reproducibility, long-term stability (> 2 months) and good repeatability.

引用

页码：1402 / 1410

页数：9

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