Strategy to Establish Embryo-Derived Pluripotent Stem Cells in Cattle

被引:5
作者
Kim, Daehwan [1 ]
Roh, Sangho [2 ]
机构
[1] Univ Calif Berkeley, Dept Bioengn & QB3, Berkeley, CA 94720 USA
[2] Seoul Natl Univ, Sch Dent, Dent Res Inst, Cellular Reprogramming & Embryo Biotechnol Lab, Seoul 08826, South Korea
关键词
bovine; embryonic stem cells; induced pluripotent stem cells; extended pluripotent stem cells; BLASTOCYST-LIKE STRUCTURES; NUCLEAR TRANSFER; SOMATIC-CELLS; GENE-EXPRESSION; BOVINE; LINES; GENERATION; DERIVATION; NANOG; NAIVE;
D O I
10.3390/ijms22095011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stem cell research is essential not only for the research and treatment of human diseases, but also for the genetic preservation and improvement of animals. Since embryonic stem cells (ESCs) were established in mice, substantial efforts have been made to establish true ESCs in many species. Although various culture conditions were used to establish ESCs in cattle, the capturing of true bovine ESCs (bESCs) has not been achieved. In this review, the difficulty of establishing bESCs with various culture conditions is described, and the characteristics of proprietary induced pluripotent stem cells and extended pluripotent stem cells are introduced. We conclude with a suggestion of a strategy for establishing true bESCs.
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页数:16
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