In ovo evaluation of FloraMax®-B11 on Marek's disease HVT vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis infection in broiler chickens

被引：45

作者：

Teague, K. D. ^{[1
]}

Graham, L. E. ^{[1
]}

Dunn, J. R. ^{[2
]}

Cheng, H. H. ^{[2
]}

Anthony, N. ^{[1
]}

Latorre, J. D. ^{[1
]}

Menconi, A. ^{[3
]}

Wolfenden, R. E. ^{[3
]}

Wolfenden, A. D. ^{[1
]}

Mahaffey, B. D. ^{[1
]}

Baxter, M. ^{[1
]}

Hernandez-Velasco, X. ^{[4
]}

Merino-Guzman, R. ^{[4
]}

Bielke, L. R. ^{[5
]}

Hargis, B. M. ^{[1
]}

Tellez, G. ^{[1
]}

机构：

[1] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA

[2] USDA ARS, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA

[3] USA Inc, Pacific Vet Grp, 2135 Creek View Dr, Fayetteville, AR 72704 USA

[4] Univ Nacl Autonoma Mexico, Dept Vet Med, Mexico City 04510, DF, Mexico

[5] Ohio State Univ, Dept Anim Sci, Wooster, OH 44691 USA

来源：

POULTRY SCIENCE | 2017年 / 96卷 / 07期

关键词：

in ovo; Marek's disease vaccine; probiotic; Salmonella enteritidis; hatchability; BACTERIA PROBIOTIC CULTURE; LYMPHOID-TISSUE; GUT MICROBIOTA; INTESTINAL MORPHOLOGY; IMMUNE-RESPONSES; LAYING HENS; FATTY-ACIDS; STRATEGIES; PERFORMANCE; HEALTH;

D O I：

10.3382/ps/pew494

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Three experiments were conducted to evaluate the effect of in ovo administration of FloraMax (R)-B11 (FM) on Marek's disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis (SE) infection in chickens. Experiment 1 consisted of 3 trials. In trials 1 and 2, d 18 White Leghorn 15I(5)x7(1) embryos were randomly distributed in 4 groups: 1) HVT vaccinated in ovo and no Marek's disease virus (MDV) challenge; 2), HVT + FM vaccinated in ovo and no MDV challenge; 3) HVT vaccinated in ovo and challenge with virulent MDV (vMDV; strain 583A); and 4), HVT + FM vaccinated in ovo and challenge with vMDV. Trial 3 was designed exactly the same as Experiment 1 but chicks were challenged with very virulent MDV (vvMDV; strains Md5 and 612). Birds were monitored until 8 wk of age, and tested for MD incidence. Experiment 2 consisted of 3 trials. In each trial, d 18 broiler embryos were injected in ovo with either saline or FM to measure hatchability and gastrointestinal bacterial composition. In Experiment 3, d 18 broiler embryos were injected in ovo with either saline or FM. All chickens that hatched were orally gavaged with SE at hatch and kept for 7 d to monitor post-hatch BW. No significant difference (P > 0.05) between MD percentage in birds vaccinated with HVT alone or HVT + FM were observed in Experiment 1. In Experiment 2, probiotic did not negatively affect hatchability, but did reduce lactose positive Gram-negative bacteria. Further, increase in BW was associated with higher villi surface area in the ileum in chickens that received the probiotic as well as a significant reduction in the SE incidence in Experiment 3. These results suggest that in ovo administration of FM does not negatively impact the ability of HVT to protect against MD or hatchability of chickens, but improves BW during the first 7 d of life and decreases SE recovery in chickens.

引用

页码：2074 / 2082

页数：9

共 85 条

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