TGF-β2 and TGF-β1 differentially regulate the odontogenic and osteogenic differentiation of mesenchymal stem cells

被引：30
作者：
Li, Jingzhi [1 ,2 ]
Ge, Lihong [1 ]
Zhao, Yuming [1 ]
Zhai, Yue [3 ]
Rao, Nanquan [4 ]
Yuan, Xiaojing [1 ]
Yang, Jie [1 ]
Li, Jing [5 ]
Yu, Shi [1 ,6 ]
机构：
[1] Peking Univ Sch & Hosp Stomatol, Dept Pediat Dent, Beijing, Peoples R China
[2] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Coll Stomatol, Dept Pediat Dent,Sch Med, Shanghai, Peoples R China
[3] Capital Med Univ, Beijing Childrens Hosp, Dept Stomatol, Beijing, Peoples R China
[4] Kunming Med Univ, Hosp Stomatol, Dept Dent Res, Kunming, Peoples R China
[5] China Japan Friendship Hosp, Dent Med Ctr, Beijing, Peoples R China
[6] Peking Univ Sch & Hosp Stomatol, Dept Pediat Dent, Zhongguancun South Ave 22, Beijing 100081, Peoples R China
来源：
ARCHIVES OF ORAL BIOLOGY | 2022年 / 135卷
关键词：
TGF-beta; 1; 2; Stem cell from apical papilla; Bone marrow stem cell; Odontogenic differentiation; Osteogenic differentiation; T-BETA-RII; APICAL PAPILLA; DENTAL-PULP; TRANSFORMING GROWTH-FACTOR-BETA-1; PULP/DENTIN REGENERATION; TISSUE FORMATION; GENE-EXPRESSION; GROWTH; OSTEOBLASTS; SCAFFOLD;
D O I：
10.1016/j.archoralbio.2022.105357
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objective: To explore the effects of transforming growth factor -beta 2 (TGF-beta 2) and TGF-beta 1 on the odontogenic and osteogenic differentiation of mesenchymal stem cells (MSCs). Design: We used lentiviral transduction to knock down TGF-beta 1 or TGF-beta 2 in stem cells from dental apical papilla (SCAPs), and to generate bone marrow mesenchymal stem cells (BMSCs) with overexpression of TGF-beta 1 or TGF-beta 2. We investigated the odontogenic and osteogenic differentiation abilities of these transductants in vitro and in vivo. Results: In vitro, TGF-beta 2 knockdown in SCAPs reduced the expression of odontoblast-related markers DSPP and DMP-1, and increased the expression of osteoblast-related markers OCN and RUNX-2. Conversely, TGF-beta 1 knockdown had the opposite effects. TGF-beta 2 overexpression promoted expression of odontoblast-related markers in BMSCs at early differentiation, but inhibited the expression of odontoblast-related markers at later stages. TGF-beta 2 overexpression attenuated expression of osteogenic-related markers in BMSCs, while TGF-beta 1 over expression enhanced odontoblast-related and osteoblast-related markers. SCAP or BMSC transductants were transplanted underneath kidneys in vivo. Masson staining showed that knockdown of TGF-beta 1, but not TGF-beta 2 promoted the expression of type I collagen in SCAPs. Immunohistochemical staining showed that TGF-beta 2 knockdown inhibited DSPP expression in SCAPs, but TGF-beta 1 knockdown had no obvious effect on DSPP expression. In vivo, TGF-beta 1 overexpression and TGF-beta 2 overexpression had no effect on the expression of type I collagen and DSPP in BMSCs. Conclusions: TGF-beta 2 promotes odontogenic differentiation of SCAPs and attenuates osteogenic differentiation of SCAPs and BMSCs. TGF-beta 1 promotes osteogenic differentiation of BMSCs and plays a complex role in regulating odontogenic differentiation of MSCs.
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页数：10
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