Restoration of spermatogenesis in infertile mice by Sertoli cell transplantation

被引：106

作者：

Shinohara, T ^{[1
]}

Orwig, KE ^{[1
]}

Avarbock, MR ^{[1
]}

Brinster, RL ^{[1
]}

机构：

[1] Univ Penn, Sch Vet Med, Dept Anim Biol, Philadelphia, PA 19104 USA

来源：

BIOLOGY OF REPRODUCTION | 2003年 / 68卷 / 03期

关键词：

developmental biology; gamete biology; male reproductive tract; Sertoli cells; spermatogenesis;

D O I：

10.1095/biolreprod.102.009977

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The niche is considered to play an important role in stem cell biology. Sertoli cells are the only somatic cells in the seminiferous tubule that closely interact with germ cells to create a favorable environment for spermatogenesis. However, little is known about how Sertoli cells develop to form the male germ line niche. We report here that Sertoli cells recovered and dissociated from testes of donor male mice can be microinjected into recipient testes, form mature seminiferous tubule structures, and support spermatogenesis. Sertoli cells from perinatal donors had a dramatically greater capacity for generating seminiferous tubules than those from adult donors. Furthermore, transplantation of wild-type Sertoli cells into infertile Steel/Steel(dickie) testes created a permissive testicular microenvironment for generating spermatogenesis and spermatozoa. Thus, our results demonstrate that the male germ line stem cell niche can be transferred between animals. in addition, the technique provides a novel tool with which to analyze spermatogenesis and might provide a mechanism for correcting fertility in males suffering from supporting cell defects.

引用

页码：1064 / 1071

页数：8

共 40 条

[1]

BELLVE AR, 1993, METHOD ENZYMOL, V225, P84

[2] Turning brain into blood: A hematopoietic fate adopted by adult neural stem cells in vivo [J].

Bjornson, CRR ;

Rietze, RL ;

Reynolds, BA ;

Magli, MC ;

Vescovi, AL .

SCIENCE, 1999, 283 (5401) :534-537

[3] SPECIAL PROLIFERATIVE SITES ARE NOT NEEDED FOR SEEDING AND PROLIFERATION OF TRANSFUSED BONE-MARROW CELLS IN NORMAL SYNGENEIC MICE [J].

BRECHER, G ;

ANSELL, JD ;

MICKLEM, HS ;

TJIO, JH ;

CRONKITE, EP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (16) :5085-5087

[4] SPERMATOGENESIS FOLLOWING MALE GERM-CELL TRANSPLANTATION [J].

BRINSTER, RL ;

ZIMMERMANN, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (24) :11298-11302

[5] GERMLINE TRANSMISSION OF DONOR HAPLOTYPE FOLLOWING SPERMATOGONIAL TRANSPLANTATION [J].

BRINSTER, RL ;

AVARBOCK, MR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (24) :11303-11307

[6]

De Kretser D. M., 1994, P1177

[7] IS THE MARROW STROMA TRANSPLANTABLE [J].

DEXTER, TM .

NATURE, 1982, 298 (5871) :222-223

[8] TRANSMEMBRANE FORM OF THE KIT LIGAND GROWTH-FACTOR IS DETERMINED BY ALTERNATIVE SPLICING AND IS MISSING IN THE SI(D) MUTANT [J].

FLANAGAN, JG ;

CHAN, DC ;

LEDER, P .

CELL, 1991, 64 (05) :1025-1035

[9]

Gondos B., 1993, SERTOLI CELL, P115

[10] Restoration of spermatogenesis by lentiviral gene transfer: Offspring from infertile mice [J].

Ikawa, M ;

Tergaonkar, V ;

Ogura, A ;

Ogonuki, N ;

Inoue, K ;

Verma, IM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (11) :7524-7529

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