The structure of the O-specific polysaccharide of the lipopolysaccharide from Acinetobacter strain 44 (DNA group 3)

被引：0
|
作者：
Vinogradov, EV
Brade, L
Brade, H
Holst, O [1 ]
机构：
[1] Leibniz Ctr Med & Biosci, Res Ctr Borstel, Div Struct Biochem, D-23845 Borstel, Germany
[2] Leibniz Ctr Med & Biosci, Res Ctr Borstel, Div Biochem Microbiol, D-23845 Borstel, Germany
来源：
POLISH JOURNAL OF CHEMISTRY | 2005年 / 79卷 / 02期
关键词：
Acinetobacter; lipopolysaccharide; O-specific polysaccharide; NMR spectroscopy;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
yExtraction of dry bacteria of Acinetobacter strain 44 (DNA group 3) by phenol/water gave a polymer that was identified by means of serological studies as S-form lipopolysaccharide (LPS). Mild acetic acid hydrolysis degraded the O-specific polysaccharide, thus, its structure was investigated by compositional analyses and NMR spectroscopy after de-O-acylation and Smith degradation of the LPS. The structure of the O-specific polysaccharides was 3)-alpha-L-RhV-(1-->2)-alpha-L-Rhap-(1-->3)-beta-D-GlcpNAc-(1-->3)-alpha-L-Rhap-(1-2 up arrow 1 alpha-L-Rhap-(1-->4)-beta-D-GlcpA-(1-->2)-alpha-L-Rhap After immunization of BALB/c mice with Acinetobacter strain 44, monoclonal antibody S48-26 (IgG(3) isotype) was obtained which reacted in Western blot with this LPS and characterized it as S-form.
引用
收藏
页码：267 / 273
页数：7
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