The structure of the O-specific polysaccharide of the lipopolysaccharide from Acinetobacter strain 44 (DNA group 3)

被引：0

作者：

Vinogradov, EV

Brade, L

Brade, H

Holst, O ^{[1
]}

机构：

[1] Leibniz Ctr Med & Biosci, Res Ctr Borstel, Div Struct Biochem, D-23845 Borstel, Germany

[2] Leibniz Ctr Med & Biosci, Res Ctr Borstel, Div Biochem Microbiol, D-23845 Borstel, Germany

来源：

POLISH JOURNAL OF CHEMISTRY | 2005年 / 79卷 / 02期

关键词：

Acinetobacter; lipopolysaccharide; O-specific polysaccharide; NMR spectroscopy;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

yExtraction of dry bacteria of Acinetobacter strain 44 (DNA group 3) by phenol/water gave a polymer that was identified by means of serological studies as S-form lipopolysaccharide (LPS). Mild acetic acid hydrolysis degraded the O-specific polysaccharide, thus, its structure was investigated by compositional analyses and NMR spectroscopy after de-O-acylation and Smith degradation of the LPS. The structure of the O-specific polysaccharides was 3)-alpha-L-RhV-(1-->2)-alpha-L-Rhap-(1-->3)-beta-D-GlcpNAc-(1-->3)-alpha-L-Rhap-(1-2 up arrow 1 alpha-L-Rhap-(1-->4)-beta-D-GlcpA-(1-->2)-alpha-L-Rhap After immunization of BALB/c mice with Acinetobacter strain 44, monoclonal antibody S48-26 (IgG(3) isotype) was obtained which reacted in Western blot with this LPS and characterized it as S-form.

引用

页码：267 / 273

页数：7

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