Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection

被引：44

作者：

Kaushik, Ajeet ^{[1
,2
]}

Solanki, Pratima R. ^{[1
]}

Kaneto, Keiichi ^{[3
]}

Kim, C. G. ^{[4
]}

Ahmad, Sharif ^{[2
]}

Malhotra, Bansi D. ^{[1
,4
]}

机构：

[1] Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

[2] Jamia Millia Islamia, Dept Chem, Mat Res Lab, New Delhi 110025, India

[3] Kyushu Inst Technol, Grad Sch Life Sci & Syst Engn, Kitakyushu, Fukuoka 8080196, Japan

[4] Chungnam Natl Univ, Ctr Nanobioengn & Spintron, Taejon 305764, South Korea

来源：

ELECTROANALYSIS | 2010年 / 22卷 / 10期

关键词：

Nanostrutured Fe3O4; Impedimetric biosensors; Cholesterol oxidase; Biosensors; FE3O4; NANOPARTICLES; ELECTRON-TRANSFER; IMMUNOSENSOR; HEMOGLOBIN; PARTICLES; FILM;

D O I：

10.1002/elan.200900468

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A nanostructured iron oxide (NanoFe(3)O(4), particle size ca. 25 nm and roughness ca. 2:1 nm) film deposited onto a hydrolyzed indium-tin-oxide (ITO) coated glass plate has been used to immobilize cholesterol oxidase (ChOx) to fabricate an impedimetric cholesterol sensor. Electrochemical studies reveal that surface charged Fe3O4 nanoparticles provide better conformation for ChOx loading resulting in enhanced electron transfer between ChOx and the electrode. Impedimetric response studies of the ChOx/NanoFe(3)O(4)/ITO bioelectrode exhibit improved linearity (2.5-400 mg/dL), low detection limit (0.25 mg/dL), fast response time (25 s), high sensitivity (86 Omega/mg dL(-1)/cm(-2)) and a low value of the Michaelis-Menten constant (K-m 0.8 mg/dL) with a regression coefficient of 0.997.

引用

页码：1045 / 1055

页数：11

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