High Specificity Binding of Lectins to Carbohydrate-Functionalized Fiber Bragg Gratings: A New Model for Biosensing Applications

被引：15

作者：

Ryu, Geunmin ^{[1
]}

Dagenais, Mario ^{[1
]}

Hurley, Matthew T. ^{[2
]}

DeShong, Philip ^{[2
]}

机构：

[1] Univ Maryland, Dept Elect & Comp Engn, College Pk, MD 20742 USA

[2] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

来源：

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS | 2010年 / 16卷 / 03期

基金：

美国国家科学基金会;

关键词：

Biosensor; Concanavalin A (ConA); carbohydrates; evanescent field; fiber Bragg grating (FBG); monolayer detection; peanut agglutinin (PNA); surface functionalization; PEANUT AGGLUTININ; ASSAY; SENSORS;

D O I：

10.1109/JSTQE.2009.2032427

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

The functionalization of an etched fiber Bragg grating was realized using a carbohydrate-siloxane conjugate. No fluorescent probes were used. Concanavalin A bound with high specificity to the glucose biosensor, but not to the lactose functionalized fiber. Conversely, peanut agglutinin bound to the lactose sensor with high specificity over its glucose counterpart. Quasi-monolayer selective binding of the lectins to the fiber sensor was inferred based on a theoretical analysis of the observed changes in the refractive index. Our results open the way to the use of unlabeled carbohydrate-based sensors for the study of the human glycome.

引用

页码：647 / 653

页数：7