Improved sensitivity for two-photon frequency-domain lifetime measurement

被引：7

作者：

Estrada, Arnold D. ^{[1
]}

Dunn, Andrew K. ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 13期

基金：

美国国家科学基金会;

关键词：

OXYGEN CONCENTRATION; FLUORESCENCE; PHOSPHORESCENCE; MICROSCOPY; MODULATION;

D O I：

10.1364/OE.18.013631

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate a method to improve the measurement sensitivity of two-photon frequency-domain lifetime measurements in poor signal to background conditions. This technique uses sinusoidal modulation of the two-photon excitation source and detection of the second harmonic of the modulation frequency that appears in the emission. Additionally, we present the mathematical model which describes how the observed phase shift and amplitude demodulation factor of two-photon phosphorescence emission are related to the phosphorescence lifetime and modulation frequency. We demonstrate the validity of the model by showing the existence of new frequency terms in the phosphorescence emission generated from the quadratic nature of two-photon absorption and by showing that the phase shift and demodulation match theory for all frequency components. (C) 2010 Optical Society of America

引用

页码：13631 / 13639

页数：9

共 50 条

[21] Frequency-domain lifetime imaging methods at unilever research
Birmingham J.J.
Journal of Fluorescence, 1997, 7 (1) : 45 - 54
[22] Frequency-domain lifetime imaging methods at Unilever research
Port Sunlight Lab, Merseyside, United Kingdom
Journal of Fluorescence, 1997, 7 (01): : 45 - 54
[23] Measurement of cerebral oxygen pressure in living mice by two-photon phosphorescence lifetime microscopy
Erlebach, Eva
Ravotto, Luca
Wyss, Matthias T.
Condrau, Jacqueline
Troxler, Thomas
Vinogradov, Sergei A.
Weber, Bruno
STAR PROTOCOLS, 2022, 3 (02):
[24] FREQUENCY-DOMAIN FLUORESCENCE LIFETIME MICROSCOPE WITH GHZ RESPONSE
XIAO, JM
KNUTTEL, A
PACKARD, BS
KNUTSON, JR
BIOPHYSICAL JOURNAL, 1994, 66 (02) : A161 - A161
[25] Time-resolved frequency-domain fluorescence lifetime imaging microscopy in the photon-counting regime
Colyer, Ryan A.
Lee, Claudia Y.
Gratton, Enrico
BIOPHYSICAL JOURNAL, 2007, : 321A - 321A
[26] Comparison of frequency-domain and time-domain fluorescence lifetime tomography
Kumar, Anand T. N.
Raymond, Scott B.
Bacskai, Brian J.
Boas, David A.
OPTICS LETTERS, 2008, 33 (05) : 470 - 472
[27] Quantum focusing and coherent control of nonresonant two-photon absorption in frequency domain
Li, Baihong
Xu, Yonggang
An, Lan
Lin, Qianlan
Zhu, Haifei
Lin, Fukun
Li, Yongfang
OPTICS LETTERS, 2014, 39 (08) : 2443 - 2446
[28] Demonstration of Nonlocal Dispersion Cancelled Two-Photon Bessel Interference in Frequency Domain
Galmes, B.
Decurey, J. -P.
Mbodji, I.
Furfaro, L.
Huy, K. Phan
Larger, L.
Dudley, J. M.
Merolla, J. -M.
2013 CONFERENCE ON LASERS AND ELECTRO-OPTICS EUROPE AND INTERNATIONAL QUANTUM ELECTRONICS CONFERENCE (CLEO EUROPE/IQEC), 2013,
[29] Time-resolved fluorescence intensity and anisotropy decays of 2,5-diphenyloxazole by two-photon excitation and frequency-domain fluorometry
Lakowicz, J.R.
Gryezynski, L.
Gryczynski, Z.
Danielsen, E.
Wirth, M.J.
Journal of Physical Chemistry, 1992, 96 (07):
[30] FREQUENCY-DOMAIN MEASUREMENT OF THE FREQUENCY STABILITY OF A MASER OSCILLATOR
TETU, M
BROUSSEAU, R
VANIER, J
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT, 1980, 29 (02) : 94 - 97

← 1 2 3 4 5 →