Reference organism selection for microwave atmospheric pressure plasma jet treatment of young coconut liquid endosperm

被引：24

作者：

Gabriel, Alonzo A. ^{[1
]}

Aba, Richard Paolo M. ^{[1
]}

Tayamora, Daniel Joshua L. ^{[1
]}

Colambo, Julius Ceasar R. ^{[1
]}

Siringan, Maria Auxilla T. ^{[2
]}

Rosario, Leo Mendel D. ^{[3
,4
]}

Tumlos, Roy B. ^{[3
,5
]}

Ramos, Henry J. ^{[3
]}

机构：

[1] Univ Philippines Diliman, Coll Home Econ, Dept Food Sci & Nutr, Quezon City 1101, Philippines

[2] Univ Philippines Diliman, Nat Sci Res Inst, Microbiol Res & Serv Lab, Quezon City 1101, Philippines

[3] Univ Philippines Diliman, Coll Sci, Natl Inst Phys, Plasma Phys Lab, Quezon City 1101, Philippines

[4] FEATI Univ, Coll Arts Sci & Educ, Dept Sci, Manila 1003, Philippines

[5] Univ Philippines, Coll Arts & Sci, Dept Phys Sci & Math, Manila 1000, Philippines

来源：

FOOD CONTROL | 2016年 / 69卷

关键词：

Coconut endosperm; Fruit beverage; Nonthermal pasteurization; Plasma jet treatment; Reference organism selection; DIELECTRIC BARRIER DISCHARGE; ESCHERICHIA-COLI; LISTERIA-MONOCYTOGENES; BACTERIAL IDENTIFICATION; SALMONELLA-ENTERICA; MATURE COCONUT; WATER; INACTIVATION; AIR; STERILIZATION;

D O I：

10.1016/j.foodcont.2016.04.034

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study identified a reference organism for the establishment of microwave atmospheric pressure plasma jet treatment of young coconut liquid endosperm. Three different, predominant spoilage bacteria were isolated from spoiled liquid endosperm and identified through 16S rDNA sequencing, prior to propagation and eventual challenge studies. Basic Local Alignment Search Tool comparisons revealed that the isolated spoilage bacteria were Klebsiella sp., Staphylococcus sp., and Kluyvera sp (%Identity 97.34-97.82%). The isolates were propagated to mid-stationary growth phase (17 h) and thereafter suspended in liquid endosperm. The inactivation rates of the spoilage isolates in liquid endosperm subjected to plasma jet at 450 and 650 W input power settings were determined and compared to those of pathogens Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes. Inactivation rates of all test organisms at 450 W (2.76-5.98 min) were significantly less (p < 0.05) than those at 650 W (1.86-3.11 min). In both power settings, S. enterica exhibited the significantly (p < 0.05) greatest resistance towards, plasma treatment, while Staphylococcus sp. exhibited the least. Heat penetration studies in both power settings revealed that the temperature of the liquid endosperm did not significantly increase and did not reach bactericidal ranges (<30 degrees C) during plasma jet treatment, hence the observed inactivation were attributed to antimicrobial species generated by the plasma. Optical emission spectroscopy revealed that these antimicrobial species included atomic oxygen (777.41 and 844.67 nm) and ultraviolet radiation (200-280 nm). Thus, this study determined S. enterica as an appropriate target organism for the establishment of plasma jet process for coconut liquid endosperm, the inactivation rate of which shall be soon established in higher scale treatment process. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：74 / 82

页数：9

共 61 条

[1]

Alave K. L., 2011, COCONUT WATER EXPORT

[2] A DYNAMIC APPROACH TO PREDICTING BACTERIAL-GROWTH IN FOOD [J].