The effect of force on thermodynamics and kinetics: unfolding single RNA molecules

被引：15
|
作者：
Tinoco, I [1 ]
Collin, D [1 ]
Li, PTX [1 ]
机构：
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2004年 / 32卷
关键词：
argininamide; laser tweezer; single RNA molecule; transactivation-responsive RNA (TAR RNA);
D O I：
10.1042/BST0320757
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have used laser tweezers to unfold single RNA molecules at room temperature and in physiological-type solvents. The forces necessary to unfold the RNAs are over the range 10-20 pN, forces that can be generated by cellular enzymes. The Gibbs free energy for the unfolding of TAR (transactivation-responsive) RNA from HIV was found to be increased after the addition of argininamide; the TAR hairpin was stabilized. The rate of unfolding was decreased and the rate of folding was increased by argininamide.
引用
收藏
页码：757 / 760
页数：4
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