The effect of force on thermodynamics and kinetics: unfolding single RNA molecules

被引：15
|
作者：
Tinoco, I [1 ]
Collin, D [1 ]
Li, PTX [1 ]
机构：
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2004年 / 32卷
关键词：
argininamide; laser tweezer; single RNA molecule; transactivation-responsive RNA (TAR RNA);
D O I：
10.1042/BST0320757
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have used laser tweezers to unfold single RNA molecules at room temperature and in physiological-type solvents. The forces necessary to unfold the RNAs are over the range 10-20 pN, forces that can be generated by cellular enzymes. The Gibbs free energy for the unfolding of TAR (transactivation-responsive) RNA from HIV was found to be increased after the addition of argininamide; the TAR hairpin was stabilized. The rate of unfolding was decreased and the rate of folding was increased by argininamide.
引用
收藏
页码：757 / 760
页数：4
相关论文
共 50 条
  • [21] Thermodynamics and Kinetics of the Pressure Unfolding of Phosphoglycerate Kinase
    Osvath, Szabolcs
    Quynh, Luu Manh
    Smeller, Laszlo
    BIOCHEMISTRY, 2009, 48 (42) : 10146 - 10150
  • [22] Fluorescence and force study of folding unfolding transitions of single titin molecules.
    Zhuang, X
    Ha, T
    Kim, HD
    Chu, S
    Labeit, S
    BIOPHYSICAL JOURNAL, 1999, 76 (01) : A9 - A9
  • [23] Stretching single molecules along unbinding and unfolding pathways with the scanning force microscope
    Samorì, B
    CHEMISTRY-A EUROPEAN JOURNAL, 2000, 6 (23) : 4249 - 4255
  • [24] A two-state kinetic model for the unfolding of single molecules by mechanical force
    Ritort, F
    Bustamante, C
    Tinoco, I
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) : 13544 - 13548
  • [25] Atomic force microscope-based single-molecule force spectroscopy of RNA unfolding
    Heus, Hans A.
    Puchner, Elias M.
    van Vugt-Jonker, Aafke J.
    Zimmermann, Julia L.
    Gaub, Hermann E.
    ANALYTICAL BIOCHEMISTRY, 2011, 414 (01) : 1 - 6
  • [26] Unfolding RNA secondary structure by force
    Hyeon, C
    Thirumalai, D
    BIOPHYSICAL JOURNAL, 2004, 86 (01) : 313A - 313A
  • [27] Force unfolding single RNAs
    Liu, F
    Tong, H
    Ou-Yang, ZC
    BIOPHYSICAL JOURNAL, 2006, 90 (06) : 1895 - 1902
  • [28] Force-driven folding and unfolding transitions in single titin molecules Single polymer strand manipulation
    Kellermayer, MSZ
    Smith, S
    Bustamante, C
    Granzier, HL
    STRUCTURE AND DYNAMICS OF CONFINED POLYMERS, 2002, 87 : 311 - 326
  • [29] Combining Temperature and Force to Study Folding of Single RNA Molecules
    Stephenson, William T.
    Santiago, Rachel
    Keller, Sean
    Tenenbaum, Scott
    Zuker, Michael
    Li, Pan T. X.
    BIOPHYSICAL JOURNAL, 2013, 104 (02) : 412A - 412A
  • [30] Unfolding Thermodynamics of an RNA Pseudoknot and its DNA Analog
    Khustsishvili, Irine
    Reiling, Calliste
    Marky, Luis A.
    BIOPHYSICAL JOURNAL, 2021, 120 (03) : 314A - 315A
12345