Assessment of genotoxic effects of benzyl derivatives by the comet assay

被引：49

作者：

Demir, Esref ^{[1
]}

Kocaoglu, Serap ^{[1
]}

Kaya, Buelent ^{[1
]}

机构：

[1] Akdeniz Univ, Fac Arts & Sci, Dept Biol, TR-07058 Antalya, Turkey

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2010年 / 48卷 / 05期

关键词：

Comet assay; Human lymphocytes; Benzyl derivatives; DNA damage; Genotoxicity; SISTER-CHROMATID EXCHANGES; HAMSTER OVARY CELLS; DNA-DAMAGE; FOOD-ADDITIVES; CHEMICALS; INDUCTION; CARCINOGENS; ABERRATION; DROSOPHILA; WORKERS;

D O I：

10.1016/j.fct.2010.02.016

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this study, different concentrations of four benzyl derivatives (benzyl alcohol, benzyl acetate, benzoic acid and benzaldehyde) used as flavour ingredients were investigated for genotoxicity in in vitro. By taking blood from two healthy people comet assay was carried on to investigate the potential health damages of benzyl derivatives. For the evaluation of genotoxic effects, the tail moment and % tail DNA in the treated chemicals were compared to the solvent control, which is distilled water. The alkaline comet assay showed significantly increased tail moment and % tail DNA at 25 and 50 mM concentrations of benzyl alcohol. Benzyl acetate increased both % tail DNA and tail moment at 50 mM concentrations. While % tail DNA was statistically increased at 10 mM and higher concentrations, tail moment has significant difference at 10 and 25 mM concentrations of benzaldehyde. Benzoic acid has apoptotic effects at the concentrations higher than 5 mM, for this reason we tested concentrations less than 5 mM (0.05, 0.1, 0.5, 1 and 5 mM). Only the highest concentration of benzoic acid increased both tail moment and % tail DNA. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.

引用

页码：1239 / 1242

页数：4

共 40 条

[1] The FEMA GRAS assessment of benzyl derivatives used as flavor ingredients [J].

Adams, TB ;

Cohen, SM ;

Doull, J ;

Feron, VJ ;

Goodman, JI ;

Marnett, LJ ;

Munro, IC ;

Portoghese, PS ;

Smith, RL ;

Waddell, WJ ;

Wagner, BM .

FOOD AND CHEMICAL TOXICOLOGY, 2005, 43 (08) :1207-1240

[2] CHROMOSOME ABERRATION AND SISTER CHROMATID EXCHANGE TEST-RESULTS WITH 42 CHEMICALS [J].

ANDERSON, BE ;

ZEIGER, E ;

SHELBY, MD ;

RESNICK, MA ;

GULATI, DK ;

IVETT, JL ;

LOVEDAY, KS .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1990, 16 :55-137

[3]

[Anonymous], 1993, NIH PUBL

[4]

[Anonymous], NIH PUBL

[5] DNA Damage in Pakistani Agricultural Workers Exposed to Mixture of Pesticides [J].

Bhalli, Javed A. ;

Ali, Tayyaba ;

Asi, M. R. ;

Khalid, Zafar M. ;

Ceppi, Marcello ;

Khan, Qaiser M. .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2009, 50 (01) :37-45

[6] Genotoxicity assessment using comet assay in Behcet's disease patients [J].

Bilgici, B ;

Bedir, A ;

Sentürk, N ;

Alvur, M ;

Aydin, F ;

Turanli, AY .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2005, 578 (1-2) :170-174

[7] KINETIC STUDIES OF THE METABOLISM OF FOREIGN ORGANIC COMPOUNDS .1. THE FORMATION OF BENZOIC ACID FROM BENZAMIDE, TOLUENE, BENZYL ALCOHOL AND BENZALDEHYDE AND ITS CONJUGATION WITH GLYCINE AND GLUCURONIC ACID IN THE RABBIT [J].

BRAY, HG ;

THORPE, WV ;

WHITE, K .

BIOCHEMICAL JOURNAL, 1951, 48 (01) :88-96

[8]

Briggs D.R., 1997, FOOD NUTR

[9] THE MUTAGENIC ACTIVITY OF SELECTED COMPOUNDS AT THE TK LOCUS - RODENT VS HUMAN-CELLS [J].

CASPARY, WJ ;

LANGENBACH, R ;

PENMAN, BW ;

CRESPI, C ;

MYHR, BC ;

MITCHELL, AD .

MUTATION RESEARCH, 1988, 196 (01) :61-81

[10] Evaluation of DNA damage in flight personnel by Comet assay [J].

Cavallo, D ;

Tomao, P ;

Marinaccio, A ;

Perniconi, B ;

Setini, A ;

Palmi, S ;

Iavicoli, S .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2002, 516 (1-2) :148-152

← 1 2 3 4 →