Comparing the Protection Imparted by Different Fraction Extracts of Garlic (Allium sativum L.) against Der p-Induced Allergic Airway Inflammation in Mice

被引：7

作者：

Hsieh, Chia-Chen ^{[1
]}

Liu, Keng-Fan ^{[2
]}

Liu, Pei-Chun ^{[3
]}

Ho, Yaw-Tsan ^{[4
]}

Li, Wei-Sung ^{[2
,5
]}

Peng, Wen-Huang ^{[2
]}

Tsai, Jen-Chieh ^{[3
]}

机构：

[1] Cheng Ching Hosp, Dept Med, Div Chest Med, 966,Sec 4,Taiwan Rd, Taichung 40764, Taiwan

[2] China Med Univ, Sch Chinese Pharmaceut Sci & Chinese Med Resource, 91 Hsueh Shih Rd, Taichung 40402, Taiwan

[3] Dayeh Univ, Dept Med Bot & Hlth Applicat, 168 Univ Rd, Chunghua 30012, Taiwan

[4] Taipei Tzu Chi Hosp, Buddhist Tzu Chi Med Fdn, Dept Emergency Med, 289 Jianguo Rd, New Taipei 23142, Taiwan

[5] Agr Res Inst Taiwan, Council Agr, Execut Yuan, Plant Pathol Div, Wufeng 41362, Taiwan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 19期

关键词：

garlic; asthma; Der p; anti-inflammatory; Th1; Th2; MURINE MODEL; TH1/TH2; CYTOKINES; ASTHMA; CELLS; IGE; EXPRESSION; RESPONSES;

D O I：

10.3390/ijms20194879

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Garlic (Allium sativum L.) has been used extensively as a food ingredient and medicinally, but the effect on asthmatic airway inflammation has not been studied in detail. We accordingly explored the protective effects exerted by various garlic fraction extracts against airway inflammation with Dermatophagoides pteronyssinus (Der p)-induced allergic asthma in vivo and in vitro. Garlic extraction was realized using n-hexane, dichloromethane, ethylacetate, n-butanol, and water in sequence to obtain different fraction extracts. Mice were orally administered different fractions (80 mg/kg) daily for four weeks. The histological results showed that the water fraction could ameliorate lung-based goblet cell hyperplasia, inflammatory cell infiltration, and mucus hypersecretion. The water fraction extracts decreased IgE and IgG1, and they decreased inflammatory cells as quantified in bronchoalveolar lavage fluid (BALF); however, they increased IgG2a in serum. Moreover, the water fraction extracts increased IFN-gamma and IL-12 (both constituting Th1 cytokines) in BALF, but they reduced IL-13, -4, and -5 (all constituting Th2 cytokines), and also inhibited the expression of IL-1 beta, IL-6, and TNF-alpha. The water fraction also inhibited the PI3K/Akt/NF-kappa B signal pathways in A549 cells. These findings suggest that water fraction extracts of garlic have a clear anti-inflammatory effect on Der p-induced allergic asthma.

引用

页数：17