Synthesis and characterization of fructooligosaccharides using levansucrase with a high concentration of sucrose

被引：8
作者：
Seo, ES
Lee, JH
Cho, JY
Seo, MY
Lee, HS
Chang, SS
Lee, HJ
Choi, JS
Kim, D [1 ]
机构：
[1] Chonnam Natl Univ, Lab Funct Carbohydrate Enzyme & Microbial Genom, Kwangju 500757, South Korea
[2] Chonnam Natl Univ, Dept Mat Chem & Biochem Engn, Kwangju 500757, South Korea
[3] Chonnam Natl Univ, Engn Res Inst, Kwangju 500757, South Korea
[4] Chonnam Natl Univ, Engn Res Inst, Kwangju 500757, South Korea
[5] Chonnam Natl Univ, Dept Phys, Kwangju 500757, South Korea
[6] Chonnam Natl Univ, Dept Mol & Biotechnol, Kwangju 500757, South Korea
[7] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea
[8] Chonnam Natl Univ, Res Inst Catalysis, Kwangju 500757, South Korea
[9] Pohang Accelerator Lab, Pohang 790784, South Korea
[10] Dongshin Univ, Biol Res Ctr Ind Accelerators, Joennam 520714, South Korea
来源：
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2004年 / 9卷 / 05期
关键词：
levansucrase; fructooligosaccharides; inhibition; insoluble glucan;
D O I：
10.1007/BF02933054
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method for synthesizing branched fructo-oligosaccha rides (BFOS) with a high concentration of sucrose (1similar to3 M) was developed using levansucrase prepared from Leuconostoc mesenteroides B-1355C. The degree of polymerization of oligosaccharides synthesized according to the present method ranged from 2 to over 15. The synthesized BFOS were stable at a pH ranges of 2 to 4 under 120degreesC. The percentage of BFOS in the reaction digest was 95.7% (excluding monosaccharides; 4.3% was levan). BFOS reduced the insoluble glucan formation by 5treptococcus sobrinus on the surfaces of glass vials or stainless steel wires in the presence of sucrose. They also reduced the growth and acid productions of S. sobrinus. Oligosaccharides can be used as sweeteners for foods such as beverages requiring thermo- and acid-stable properties and as potential inhibitors of dental caries.
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收藏
页码：339 / 344
页数：6
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