VISLISI trial, a prospective clinical study allowing identification of a new metalloprotease and putative virulence factor from Staphylococcus lugdunensis

被引：30

作者：

Argemi, X. ^{[1
,2
]}

Prevost, G. ^{[2
,3
]}

Riegel, P. ^{[2
,3
]}

Keller, D. ^{[3
]}

Meyer, N. ^{[4
]}

Baldeyrou, M. ^{[1
]}

Douiri, N. ^{[1
]}

Lefebvre, N. ^{[1
]}

Meghit, K. ^{[5
]}

Oustau, C. Ronde ^{[6
]}

Christmann, D. ^{[1
,2
]}

Cianferani, S. ^{[7
]}

Strub, J. M. ^{[7
]}

Hansmann, Y. ^{[1
,2
]}

机构：

[1] Hop Univ, Malad Infect & Trop, Strasbourg, France

[2] Univ Strasbourg, CHRU Strasbourg, VBP EA7290, FMTS,Inst Bacteriol, 3 Rue Koeberle, Strasbourg, France

[3] Hop Univ, Lab Microbiol, Strasbourg, France

[4] Hop Univ, Serv Sante Publ, Strasbourg, France

[5] Hop Univ, Immunol Clin, Strasbourg, France

[6] Hop Univ, Serv Chirurg Orthoped, CCOM, Strasbourg, France

[7] UMR 7178 CNRS UdS, Inst Pluridiscipl naire Hubert Curien, Lab Spectrometrie Masse Bioorgan, Dept Sci Analyt, Strasbourg, France

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2017年 / 23卷 / 05期

关键词：

Bone and joint infections; Mass spectrometry; Metalloprotease; Staphylococcus lugdunensis; Virulence factors; COAGULASE-NEGATIVE STAPHYLOCOCCI; INFECTIOUS-DISEASES SOCIETY; MICROBIOLOGICAL CHARACTERISTICS; PRACTICE GUIDELINES; AUREUS; DIAGNOSIS; MATRIX; BONE; BIOFILMS;

D O I：

10.1016/j.cmi.2016.12.018

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objective: Staphylococcus lugdunensis is a coagulase-negative staphylococcus that displays an unusually high virulence rate close to that of Staphylococcus aureus. It also shares phenotypic properties with S. aureus and several studies found putative virulence factors. The objective of the study was to describe the clinical manifestations of S. lugdunensis infections and investigate putative virulence factors. Method: We conducted a prospective study from November 2013 to March 2016 at the University Hospital of Strasbourg. Putative virulence factors were investigated by clumping factor detection, screening for proteolytic activity, and sequence analysis using tandem nano-liquid chromatography-mass spectrometry. Results: In total, 347 positive samples for S. lugdunensis were collected, of which 129 (37.2%) were from confirmed cases of S. lugdunensis infection. Eighty-one of these 129 patients were included in the study. Bone and prosthetic joints (PJI) were the most frequent sites of infection (n = 28; 34.6%) followed by skin and soft tissues (n = 23; 28.4%). We identified and purified a novel protease secreted by 50 samples (61.7%), most frequently associated with samples from deep infections and PJI (pr 0.97 and pr 0.91, respectively). Protease peptide sequencing by nano-liquid chromatography-mass spectrometry revealed a novel protease bearing 62.42% identity with ShpI, a metalloprotease secreted by Staphylococcus hyicus. Conclusion: This study confirms the pathogenicity of S. lugdunensis, particularly in bone and PJI. We also identified a novel metalloprotease called lugdulysin that may contribute to virulence. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

引用

页码：334.e1 / 334.e8

页数：8

共 34 条

[1] Implementation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase-Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis [J].