Highly narrow nanogap-containing Au@Au core-shell SERS nanoparticles: size-dependent Raman enhancement and applications in cancer cell imaging

被引:84
作者
Hu, Chongya [1 ]
Shen, Jianlei [2 ]
Yan, Juan [3 ,5 ]
Zhong, Jian [3 ,5 ]
Qin, Weiwei [2 ]
Liu, Rui [3 ]
Aldalbahi, Ali [4 ]
Zuo, Xiaolei [2 ]
Song, Shiping [2 ]
Fan, Chunhai [2 ]
He, Dannong [1 ,3 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Mat Sci & Engn, Shanghai 200240, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol & Bioimaging Ctr, Shanghai 201800, Peoples R China
[3] Natl Engn Res Ctr Nanotechnol, Shanghai 200241, Peoples R China
[4] King Saud Univ, Chem Dept, POB 2455, Riyadh 11451, Saudi Arabia
[5] Shanghai Ocean Univ, Shanghai 201306, Peoples R China
基金
美国国家科学基金会; 上海市科技启明星计划;
关键词
SINGLE-MOLECULE SERS; GOLD NANOPARTICLES; HOT-SPOTS; SCATTERING; DNA; CYTOTOXICITY; LOCALIZATION; NANOPROBES; NANOTAGS; UNIFORM;
D O I
10.1039/c5nr06919j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Cellular imaging technologies employing metallic surface-enhanced Raman scattering (SERS) tags have gained much interest toward clinical diagnostics, but they are still suffering from poor controlled distribution of hot spots and reproducibility of SERS signals. Here, we report the fabrication and characterization of high narrow nanogap-containing Au@Au core-shell SERS nanoparticles (GCNPs) for the identification and imaging of proteins overexpressed on the surface of cancer cells. First, plasmonic nanostructures are made of gold nanoparticles (similar to 15 nm) coated with gold shells, between which a highly narrow and uniform nanogap (similar to 1.1 nm) is formed owing to polyA anchored on the Au cores. The well controlled distribution of Raman reporter molecules, such as 4,4'-dipyridyl (44DP) and 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), are readily encoded in the nanogap and can generate strong, reproducible SERS signals. In addition, we have investigated the size-dependent SERS activity of GCNPs and found that with the same laser wavelength, the Raman enhancement discriminated between particle sizes. The maximum Raman enhancement was achieved at a certain threshold of particle size (similar to 76 nm). High narrow nanogap-containing Au@Au core-shell SERS tags (GCTs) were prepared via the functionalization of hyaluronic acid (HA) on GCNPs, which recognized the CD44 receptor, a tumor-associated surface biomarker. And it was shown that GCTs have a good targeting ability to tumour cells and promising prospects for multiplex biomarker detection.
引用
收藏
页码:2090 / 2096
页数:7
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