Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice

被引：50
作者：
Zhu, Huachen [1 ,2 ,3 ]
Wang, Jia [1 ,2 ,3 ]
Wang, Pui [1 ,2 ,3 ]
Song, Wenjun [1 ,2 ,3 ]
Zheng, Zuoyi [3 ]
Chen, Rirong [3 ]
Guo, Kunyuan [3 ]
Zhang, Taixing [3 ]
Peiris, Joseph S. M. [1 ,2 ]
Chen, Honglin [1 ,2 ,3 ]
Guan, Yi [1 ,2 ,3 ]
机构：
[1] Univ Hong Kong, Li Ka Shing Fac Med, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Li Ka Shing Fac Med, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
[3] Shantou Univ, Coll Med, Int Inst Infect & Immun, Shantou 515031, Guangdong, Peoples R China
来源：
VIROLOGY | 2010年 / 401卷 / 01期
基金：
美国国家卫生研究院;
关键词：
Influenza virus; Pathogenicity; Virulence; PB2; 627; residue; Temperature-sensitivity; INFLUENZA-A VIRUSES; HOST-RANGE; MOLECULAR-BASIS; AMINO-ACID; POLYMERASE; REPLICATION; DETERMINANT; ADAPTATION; CELLS;
D O I：
10.1016/j.virol.2010.02.024
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A lysine at the 627 position (627K) of PB2 protein of influenza virus has been recognized as a determinant for host adaptation and a virulent element for some influenza viruses. While seasonal influenza viruses exclusively contained 627K, the pandemic (H1N1) 2009 possessed a glutamic acid (627E), even after circulation in humans for more than 6 months. To explore the potential role of E627K substitution in PB2 in the pandemic (H1N1) 2009 virus, we compared pathogenicity and growth properties between a recombinant virus containing 627K PB2 gene and the parental A/California/4/2009 strain containing 627E. Our results showed that substitution of 627K in PB2 gene does not confer higher virulence and growth rate for the pandemic (H1N1) 2009 virus in mice and cell culture respectively, suggesting 627K is not required for human adaptation of the pandemic (H1N1) 2009 virus. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：1 / 5
页数：5
相关论文
共 19 条
  • [1] Differential polymerase activity in avian and mammalian cells determines host range of influenza virus
    Gabriel, G.
    Abram, M.
    Keiner, B.
    Wagner, R.
    Klenk, H.-D.
    Stech, J.
    [J]. JOURNAL OF VIROLOGY, 2007, 81 (17) : 9601 - 9604
  • [2] Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses
    Hatta, M
    Gao, P
    Halfmann, P
    Kawaoka, Y
    [J]. SCIENCE, 2001, 293 (5536) : 1840 - 1842
  • [3] Growth of H5N1 influenza a viruses in the upper respiratory tracts of mice
    Hatta, Masato
    Hatta, Yasuko
    Kim, Jin Hyun
    Watanabe, Shinji
    Shinya, Kyoko
    Lien, Tung Nguyen Phuong Song
    Le, Quynh Mai
    Kawaoka, Yoshihiro
    [J]. PLOS PATHOGENS, 2007, 3 (10) : 1374 - 1379
  • [4] A DNA transfection system for generation of influenza A virus from eight plasmids
    Hoffmann, E
    Neumann, G
    Kawaoka, Y
    Hobom, G
    Webster, RG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) : 6108 - 6113
  • [5] Universal primer set for the full-length amplification of all influenza A viruses
    Hoffmann, E
    Stech, J
    Guan, Y
    Webster, RG
    Perez, DR
    [J]. ARCHIVES OF VIROLOGY, 2001, 146 (12) : 2275 - 2289
  • [6] In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses
    Itoh, Yasushi
    Shinya, Kyoko
    Kiso, Maki
    Watanabe, Tokiko
    Sakoda, Yoshihiro
    Hatta, Masato
    Muramoto, Yukiko
    Tamura, Daisuke
    Sakai-Tagawa, Yuko
    Noda, Takeshi
    Sakabe, Saori
    Imai, Masaki
    Hatta, Yasuko
    Watanabe, Shinji
    Li, Chengjun
    Yamada, Shinya
    Fujii, Ken
    Murakami, Shin
    Imai, Hirotaka
    Kakugawa, Satoshi
    Ito, Mutsumi
    Takano, Ryo
    Iwatsuki-Horimoto, Kiyoko
    Shimojima, Masayuki
    Horimoto, Taisuke
    Goto, Hideo
    Takahashi, Kei
    Makino, Akiko
    Ishigaki, Hirohito
    Nakayama, Misako
    Okamatsu, Masatoshi
    Takahashi, Kazuo
    Warshauer, David
    Shult, Peter A.
    Saito, Reiko
    Suzuki, Hiroshi
    Furuta, Yousuke
    Yamashita, Makoto
    Mitamura, Keiko
    Nakano, Kunio
    Nakamura, Morio
    Brockman-Schneider, Rebecca
    Mitamura, Hiroshi
    Yamazaki, Masahiko
    Sugaya, Norio
    Suresh, M.
    Ozawa, Makoto
    Neumann, Gabriele
    Gern, James
    Kida, Hiroshi
    [J]. NATURE, 2009, 460 (7258) : 1021 - U110
  • [7] Host-range determinants on the PB2 protein of influenza A viruses control the interaction between the viral polymerase and nucleoprotein in human cells
    Labadie, Karine
    Afonso, Emmanuel Dos Santos
    Rameix-Welti, Marie-Anne
    van der Werf, Sylvie
    Naffakh, Nadia
    [J]. VIROLOGY, 2007, 362 (02) : 271 - 282
  • [8] Single mutation at the amino acid position 627 of PB2 that leads to increased virulence of an H5N1 avian influenza virus during adaptation in mice can be compensated by multiple mutations at other sites of PB2
    Li, Junwei
    Ishaq, Musarat
    Prudence, Mabiala
    Xi, Xiao
    Hu, Tao
    Liu, Qingzhen
    Guo, Deyin
    [J]. VIRUS RESEARCH, 2009, 144 (1-2) : 123 - 129
  • [9] PB2 Protein of a Highly Pathogenic Avian Influenza Virus Strain A/chicken/Yamaguchi/7/2004 (H5N1) Determines Its Replication Potential in Pigs
    Manzoor, Rashid
    Sakoda, Yoshihiro
    Nomura, Naoki
    Tsuda, Yoshimi
    Ozaki, Hiroichi
    Okamatsu, Masatoshi
    Kida, Hiroshi
    [J]. JOURNAL OF VIROLOGY, 2009, 83 (04) : 1572 - 1578
  • [10] Recent H5N1 avian Influenza A virus increases rapidly in virulence to mice after a single passage in mice
    Mase, Masaji
    Tanimura, Nobuhiko
    Imada, Tadao
    Okamatsu, Masatoshi
    Tsukamoto, Kenji
    Yamaguchi, Shigeo
    [J]. JOURNAL OF GENERAL VIROLOGY, 2006, 87 : 3655 - 3659
12