In Vitro Studies on Mg-Zn-Sn-Based Alloys Developed as a New Kind of Biodegradable Metal

被引:12
作者
Wen, Yafeng [1 ]
Liu, Qingshan [2 ]
Zhao, Weikang [1 ]
Yang, Qiming [1 ]
Wang, Jingfeng [2 ]
Jiang, Dianming [1 ,3 ]
机构
[1] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthopaed, 1 Youyi Rd, Chongqing 400016, Peoples R China
[2] Chongqing Univ, Coll Mat Sci & Engn, Natl Engn Res Ctr Magnesium Alloys, 174 Shapingba Main St, Chongqing 400044, Peoples R China
[3] Chongqing Med Univ, Affiliated Hosp 3, Dept Orthopaed, 1 Shuanghu Rd, Chongqing 401120, Peoples R China
基金
中国国家自然科学基金;
关键词
Mg-Zn-Sn alloy; corrosion behavior; mechanical properties; biocompatibility; osteoinductive activity; STRONTIUM RANELATE; MECHANICAL-PROPERTIES; CORROSION BEHAVIOR; HYDROXYAPATITE BIOCERAMICS; MAGNESIUM ALLOYS; STEM-CELL; MICROSTRUCTURE; OSTEOBLAST; SR; CYTOCOMPATIBILITY;
D O I
10.3390/ma14071606
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Mg-Zn-Sn-based alloys are widely used in the industrial field because of their low-cost, high-strength and heat-resistant characteristics. However, their application in the biomedical field has been rarely reported. In the present study, biodegradable Mg-1Zn-1Sn and Mg-1Zn-1Sn-0.2Sr alloys were fabricated. Their microstructure, surface characteristics, mechanical properties and bio-corrosion properties were carried out using an optical microscope (OM), X-ray diffraction (XRD), electron microscopy (SEM), mechanical testing, electrochemical and immersion test. The cell viability and morphology were studied by cell counting kit-8 (CCK-8) assay, live/dead cell assay, confocal laser scanning microscopy (CLSM) and SEM. The osteogenic activity was systematically investigated by alkaline phosphatase (ALP) assay, Alizarin Red S (ARS) staining, immunofluorescence staining and quantitative real time-polymerase chain reaction (qRT-PCR). The results showed that a small amount of strontium (Sr) (0.2 wt.%) significantly enhanced the corrosion resistance of the Mg-1Zn-1Sn alloy by grain refinement and decreasing the corrosion current density. Meanwhile, the mechanical properties were also improved via the second phase strengthening. Both Mg-1Zn-1Sn and Mg-1Zn-1Sn-0.2Sr alloys showed excellent biocompatibility, significantly promoted cell proliferation, adhesion and spreading. Particularly, significant increases in ALP activity, ARS staining, type I collagen (COL-I) expression as well as the expressions of three osteogenesis-related genes (runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (Bglap)) were observed for the Mg-1Zn-1Sn-0.2Sr group. In summary, this study demonstrated that Mg-Zn-Sn-based alloy has great application potential in orthopedics and Sr is an ideal alloying element of Mg-Zn-Sn-based alloy, which optimizes its corrosion resistance, mechanical properties and osteoinductive activity.
引用
收藏
页数:18
相关论文
共 48 条
  • [1] [Anonymous], 2013, STANDARD TEST METHOD, DOI DOI 10.1520/E0008
  • [2] ASTM International, 2015, G10289 ASTM ITN
  • [3] ASTM International, 2009, E909 ASTM INT
  • [4] Corrosion mechanism applicable to biodegradable magnesium implants
    Atrens, Andrej
    Liu, Ming
    Abidin, Nor Ishida Zainal
    [J]. MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, 2011, 176 (20): : 1609 - 1636
  • [5] Strontium Ranelate affects signaling from mechanically-stimulated osteocytes towards osteoclasts and osteoblasts
    Bakker, Astrid D.
    Zandieh-Doulabi, Behrouz
    Klein-Nulend, Jenneke
    [J]. BONE, 2013, 53 (01) : 112 - 119
  • [6] Development of magnesium-based biodegradable metals with dietary trace element germanium as orthopaedic implant applications
    Bian, Dong
    Zhou, Weirui
    Deng, Jiuxu
    Liu, Yang
    Li, Wenting
    Chu, Xiao
    Xiu, Peng
    Cai, Hong
    Kou, Yuhui
    Jiang, Baoguo
    Zheng, Yufeng
    [J]. ACTA BIOMATERIALIA, 2017, 64 : 421 - 436
  • [7] Biocompatibility and biodegradability of Mg-Sr alloys: The formation of Sr-substituted hydroxyapatite
    Bornapour, M.
    Muja, N.
    Shum-Tim, P.
    Cerruti, M.
    Pekguleryuz, M.
    [J]. ACTA BIOMATERIALIA, 2013, 9 (02) : 5319 - 5330
  • [8] Strontium-substituted hydroxyapatite coatings synthesized by pulsed-laser deposition: In vitro osteoblast and osteoclast response
    Capuccini, C.
    Torricelli, P.
    Sima, F.
    Boanini, E.
    Ristoscu, C.
    Bracci, B.
    Socol, G.
    Fini, M.
    Mihailescu, I. N.
    Bigi, A.
    [J]. ACTA BIOMATERIALIA, 2008, 4 (06) : 1885 - 1893
  • [9] Recent advances on the development of magnesium alloys for biodegradable implants
    Chen, Yongjun
    Xu, Zhigang
    Smith, Christopher
    Sankar, Jag
    [J]. ACTA BIOMATERIALIA, 2014, 10 (11) : 4561 - 4573
  • [10] Effects of minor Sr addition on microstructure, mechanical and bio-corrosion properties of the Mg-5Zn based alloy system
    Cheng, Meixin
    Chen, Jihua
    Yan, Hongge
    Su, Bin
    Yu, Zhaohui
    Xia, Weijun
    Gong, Xiaole
    [J]. JOURNAL OF ALLOYS AND COMPOUNDS, 2017, 691 : 95 - 102