Improved Imaging of Magnetically Labeled Cells Using Rotational Magnetomotive Optical Coherence Tomography

被引：7

作者：

Cimalla, Peter ^{[1
,2
]}

Walther, Julia ^{[1
,3
]}

Mueller, Claudia ^{[4
]}

Almedawar, Seba ^{[2
]}

Rellinghaus, Bernd ^{[5
]}

Wittig, Dierk ^{[4
]}

Ader, Marius ^{[2
]}

Karl, Mike O. ^{[2
,6
]}

Funk, Richard H. W. ^{[4
]}

Brand, Michael ^{[2
]}

Koch, Edmund ^{[1
]}

机构：

[1] Tech Univ Dresden, Fac Med Carl Gustav Carus, Anesthesiol & Intens Care Med Clin Sensoring & Mo, D-01307 Dresden, Germany

[2] Tech Univ Dresden, DFG Ctr Regenerat Therapies Dresden CRTD, D-01307 Dresden, Germany

[3] Tech Univ Dresden, Fac Med Carl Gustav Carus, Dept Med Phys & Biomed Engn, D-01307 Dresden, Germany

[4] Tech Univ Dresden, Fac Med Carl Gustav Carus, Inst Anat, D-01307 Dresden, Germany

[5] Leibniz Inst Solid State & Mat Res, Inst Metall Mat, D-01069 Dresden, Germany

[6] German Ctr Neurodegenerat Dis DZNE, D-01307 Dresden, Germany

来源：

APPLIED SCIENCES-BASEL | 2017年 / 7卷 / 05期

关键词：

optical coherence tomography; cell imaging; dynamic contrast agents; magnetic particles; laser speckle; fluorescence microscopy; ROYAL-COLLEGE; SURGEONS RAT; RETINA; TRANSPLANTATION; NANOPARTICLES; PRESERVATION; NANOSTARS; CORES;

D O I：

10.3390/app7050444

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this paper, we present a reliable and robust method for magnetomotive optical coherence tomography (MM-OCT) imaging of single cells labeled with iron oxide particles. This method employs modulated longitudinal and transverse magnetic fields to evoke alignment and rotation of anisotropic magnetic structures in the sample volume. Experimental evidence suggests that magnetic particles assemble themselves in elongated chains when exposed to a permanent magnetic field. Magnetomotion in the intracellular space was detected and visualized by means of 3D OCT as well as laser speckle reflectometry as a 2D reference imaging method. Our experiments on mesenchymal stem cells embedded in agar scaffolds show that the magnetomotive signal in rotational MM-OCT is significantly increased by a factor of similar to 3 compared to previous pulsed MM-OCT, although the solenoid's power consumption was 16 times lower. Finally, we use our novel method to image ARPE-19 cells, a human retinal pigment epithelium cell line. Our results permit magnetomotive imaging with higher sensitivity and the use of low power magnetic fields or larger working distances for future three-dimensional cell tracking in target tissues and organs.

引用

页数：10

共 17 条

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