Molecular typing of isolates of the fish pathogen, Flavobacterium columnare, by single-strand conformation polymorphism analysis

被引：42

作者：

Olivares-Fuster, Oscar

Shoemaker, Craig A.

Klesius, Phillip H.

Arias, Covadonga R.

机构：

[1] Auburn Univ, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA

[2] USDA ARS, Aquat Anim Hlth Res Lab, Auburn, AL USA

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 269卷 / 01期

关键词：

Flavobacterium columnare; SSCP; 16S rRNA gene; ISR; genotyping;

D O I：

10.1111/j.1574-6968.2006.00605.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Flavobacterium columnare intraspecies diversity was revealed by analyzing the 16S rRNA gene and the 16S-23S internal spacer region (ISR). Standard restriction fragment length polymorphism (RFLP) of these sequences was compared with single-strand conformation polymorphism (SSCP). Diversity indexes showed that both 16S-SSCP and ISR-SSCP improved resolution (D >= 0.9) when compared with standard RFLP. ISR-SSCP offered a simpler banding pattern than 16S-SSCP while providing high discrimination between isolates. SSCP analysis of rRNA genes proved to be a simple, rapid, and cost-effective method for routine fingerprinting of F. columnare.

引用

页码：63 / 69

页数：7

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