Molecular typing of isolates of the fish pathogen, Flavobacterium columnare, by single-strand conformation polymorphism analysis

被引:42
作者
Olivares-Fuster, Oscar
Shoemaker, Craig A.
Klesius, Phillip H.
Arias, Covadonga R.
机构
[1] Auburn Univ, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA
[2] USDA ARS, Aquat Anim Hlth Res Lab, Auburn, AL USA
关键词
Flavobacterium columnare; SSCP; 16S rRNA gene; ISR; genotyping;
D O I
10.1111/j.1574-6968.2006.00605.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Flavobacterium columnare intraspecies diversity was revealed by analyzing the 16S rRNA gene and the 16S-23S internal spacer region (ISR). Standard restriction fragment length polymorphism (RFLP) of these sequences was compared with single-strand conformation polymorphism (SSCP). Diversity indexes showed that both 16S-SSCP and ISR-SSCP improved resolution (D >= 0.9) when compared with standard RFLP. ISR-SSCP offered a simpler banding pattern than 16S-SSCP while providing high discrimination between isolates. SSCP analysis of rRNA genes proved to be a simple, rapid, and cost-effective method for routine fingerprinting of F. columnare.
引用
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页码:63 / 69
页数:7
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