Iron oxide-chitosan hybrid nanobiocomposite based nucleic acid sensor for pyrethroid detection

被引：51
作者：
Kaushik, Ajeet [1 ,2 ]
Solanki, Pratima R. [2 ]
Ansari, Anees A. [2 ]
Malhotra, Bansi D. [2 ]
Ahmad, Sharif [1 ]
机构：
[1] Jamia Millia Islamia, Dept Chem, Mat Res Lab, New Delhi 110025, India
[2] Natl Phys Lab, Ctr Biomol Elect, Dept Sci & Technol, New Delhi 110012, India
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2009年 / 46卷 / 02期
关键词：
Biosensor; Biocatalysis; Biopolymers; Fe3O4; nanoparticles; Nanobiocomposite; Electrochemical detection; POLYVINYL SULFONATE FILM; MODIFIED ELECTRODE; COMPOSITE FILM; IN-VITRO; DNA; NANOPARTICLES; PERMETHRIN; CYPERMETHRIN; IMMOBILIZATION; PESTICIDES;
D O I：
10.1016/j.bej.2009.04.021
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Nucleic acid sensor has been fabricated via immobilization of single standard calf thymus deoxyribose nucleic acid (ssCT-DNA) onto chitosan (CH)-iron oxide (Fe3O4) nanoparticles based hybrid nanobiocomposite film deposited onto indium-tin-oxide (ITO) coated glass for pyrethroids [cypermehtim (CM) and permethrin (PM)] detection. The ssCT-DNA immobilized onto CH-Fe3O4 nanocomposite/ITO electrode has been characterized using scanning electron microscopy (SEM), UV-visible, Fourier transform infrared (FTIR) spectroscopy, electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) techniques. This disposable ssCT-DNA/CH-Fe3O4 nanobiocomposite/ITO bioelectrode is stable for about two months under refrigerated conditions and can detect CM (0.0025-2 ppm) and PM (1-300 ppm) using DPV technique within 25s and 40s. respectively. (C) 2009 Published by Elsevier B.V.
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页码：132 / 140
页数：9
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