Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression

被引：113

作者：

Sheludko, Y. V. ^{[1
]}

Sindarovska, Y. R. ^{[1
]}

Gerasymenko, I. M. ^{[1
]}

Bannikova, M. A. ^{[1
]}

Kuchuk, N. V. ^{[1
]}

机构：

[1] Inst Cell Biol & Genet Engn, UA-03413 Kiev, Ukraine

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2007年 / 96卷 / 03期

关键词：

Nicotiana benthamiana; Nicotiana excelsior; Agrobacterium; transient expression; GFP;

D O I：

10.1002/bit.21075

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Agrobacterium-mediated transient expression may be regarded as a promising method for inexpensive large-scale production of recombinant proteins. We optimized the protocol of transient expression in Nicotiana benthamiana and compared six Australian species of Nicotiana as hosts for transient expression. The transient expression of GFP under 35S CaMV promoter was observed in all species tested, although the GFP content in leaves of N. benthamiana, N. exigua, and N. excelsior was significantly higher (3.8, 3.7, and 2.0% TSP, respectively). Usage of viral-based expression system resulted in considerable increase of GFP accumulation in N. excelsior and N. benthamiana (63.5 and 16.2% TSP, respectively). We displayed that N. excelsior has the best characteristics in regard to biomass yield as well as GFP accumulation level for both types of the expression cassettes tested.

引用

页码：608 / 614

页数：7

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