Species identification and antifungal susceptibility testing of Aspergillus strains isolated from patients with otomycosis in northern China

被引：21

作者：

Jing, Ran ^{[1
,2
,3
,4
]}

Yang, Wen-Hang ^{[1
,2
,3
,4
]}

Xiao, Meng ^{[1
,2
,4
]}

Li, Ying ^{[5
]}

Zou, Gui-Ling ^{[6
]}

Wang, Cheng-Ying ^{[7
]}

Li, Xiu-Wen ^{[8
]}

Xu, Ying-Chun ^{[1
,2
,4
]}

Hsueh, Po-Ren ^{[9
,10
]}

机构：

[1] Chinese Acad Med Sci, Dept Lab Med, Peking Union Med Coll Hosp, State Key Lab Complex Severe & Rare Dis, Beijing, Peoples R China

[2] Peking Union Med Coll, Beijing, Peoples R China

[3] Chinese Acad Med Sci, Grad Sch, Beijing, Peoples R China

[4] Beijing Key Lab Mech Res & Precis Diag Invas Fung, Beijing, Peoples R China

[5] Capital Med Univ, Xuanwu Hosp, Dept Clin Lab, Beijing, Peoples R China

[6] Harbin Med Univ, Affiliated Hosp 4, Harbin, Heilongjiang, Peoples R China

[7] Daqing Oilfield Gen Hosp, Daqing, Heilongjiang, Peoples R China

[8] Mudanjiang First Peoples Hosp, Mudanjiang, Heilongjiang, Peoples R China

[9] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Dept Lab Med, Coll Med, Taipei, Taiwan

[10] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Dept Internal Med, Coll Med, Taipei, Taiwan

来源：

JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION | 2022年 / 55卷 / 02期

关键词：

Otomycosis; Species identification; Aspergillus welwitschiae; Antifungal susceptibility testing; Northern China; CLINICAL-FEATURES; VORICONAZOLE;

D O I：

10.1016/j.jmii.2021.03.011

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background/Purpose: There are limited studies on species distribution and suscep-tibility profiles of Aspergillus strains isolated from patients with otomycosis in China.Methods: A total of 69 confirmed Aspergillus species isolates were obtained from ear swabs of patients diagnosed with otomycosis from 2017 to 2018 in northern China. Identification of these Aspergillus isolates at the species level was performed using conventional morphological methods and MALDI-TOF MS in combination with molecular sequencing, and in vitro suscepti-bility to nine antifungal agents was evaluated using the Sensititre YeastOne system.Results: The Aspergillus section Nigri had the greatest distribution of Aspergillus isolates. A. welwitschiae (n = 25) was the most predominant isolate in section Nigri, followed by A. tu-bingensis (n = 12) and A. niger (n = 11). Other Aspergillus species were also isolated, including A. terreus (n = 11), A. flavus/A. oryzae (n = 8), and A. fumigatus (n = 2). Ampho-tericin B, posaconazole, and echinocandins were highly in vitro active against all the isolates tested. 2.9% (2/69) of the isolates were resistant to azoles in our study, including one A. niger isolate with a high MIC value for itraconazole (ITR) (16 mg/L) and one A. tubingensis isolate cross-resistant to both voriconazole (VOR) (MIC >8 mg/L) and ITR (MIC >16 mg/L). One A. wel-witschiae and one A. niger isolate both had increased MIC values of 4 mg/L against VOR.Conclusions: A. welwitschiae was the most prevalent Aspergillus species isolated from pa-tients with otomycosis. Our findings also indicated that the azole-resistant Aspergillus section Nigri should be utilized to guide clinical medication for Otomycosis.Copyright (c) 2021, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

引用

页码：282 / 290

页数：9

共 34 条

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